PMID- 22560110 OWN - NLM STAT- MEDLINE DCOM- 20120914 LR - 20120515 IS - 1873-2534 (Electronic) IS - 0165-2427 (Linking) VI - 147 IP - 1-2 DP - 2012 Jun 15 TI - The immunoglobulin light chain locus of the turkey, Meleagris gallopavo. PG - 44-50 LID - 10.1016/j.vetimm.2012.04.018 [doi] AB - To date, most jawed vertebrate species encode more than one immunoglobulin light (IgL) chain isotypes. It has been shown that several bird species (chickens, white Pekin or domestic duck, and zebra finches) exclusively express lambda isotype. We analyze here the genomic organization of another bird species turkey IgL genes based on the recently released genome data. The turkey IgL locus located on chromosome 17 spans approximately 75.2kb and contains a single functional V(lambda) gene, twenty V(lambda) pseudogenes, and a single functional J(lambda)-C(lambda) block. These data suggest that the genomic organization of bird IgL chain genes seems to be conserved. Ten cDNA clones from turkey Iglambda chain containing almost full-length V(lambda), J(lambda) and C(lambda) segments were acquired. The comparison of V(lambda) cDNA sequences to all the germline V(lambda) segments suggests that turkey species may be generating IgL chain diversity by gene conversion and somatic hypermutation like the chicken. This study provides insights into the immunoglobulin light chain genes in another bird species. CI - Copyright (c) 2012. Published by Elsevier B.V. FAU - Bao, Yonghua AU - Bao Y AD - Department of Basic Immunology, Xinxiang Medical University, Jinsui Street, Xinxiang 453003, PR China. baoyonghua2005@yahoo.com.cn FAU - Wu, Sun AU - Wu S FAU - Zang, Yunlong AU - Zang Y FAU - Wang, Hui AU - Wang H FAU - Song, Xiangfeng AU - Song X FAU - Xu, Chunyang AU - Xu C FAU - Xie, Bohong AU - Xie B FAU - Guo, Yongchen AU - Guo Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120420 PL - Netherlands TA - Vet Immunol Immunopathol JT - Veterinary immunology and immunopathology JID - 8002006 RN - 0 (Immunoglobulin Constant Regions) RN - 0 (Immunoglobulin Joining Region) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - *Genes, Immunoglobulin Light Chain MH - Genetic Loci MH - Immunoglobulin Constant Regions/genetics MH - Immunoglobulin Joining Region/genetics MH - Molecular Sequence Data MH - Turkeys/*immunology EDAT- 2012/05/09 06:00 MHDA- 2012/09/15 06:00 CRDT- 2012/05/08 06:00 PHST- 2011/06/18 00:00 [received] PHST- 2012/03/23 00:00 [revised] PHST- 2012/04/12 00:00 [accepted] PHST- 2012/05/08 06:00 [entrez] PHST- 2012/05/09 06:00 [pubmed] PHST- 2012/09/15 06:00 [medline] AID - S0165-2427(12)00119-5 [pii] AID - 10.1016/j.vetimm.2012.04.018 [doi] PST - ppublish SO - Vet Immunol Immunopathol. 2012 Jun 15;147(1-2):44-50. doi: 10.1016/j.vetimm.2012.04.018. Epub 2012 Apr 20.