PMID- 22561674
OWN - NLM
STAT- MEDLINE
DCOM- 20130214
LR  - 20120924
IS  - 1940-2465 (Electronic)
IS  - 1066-8969 (Linking)
VI  - 20
IP  - 5
DP  - 2012 Oct
TI  - Fluorescence in situ hybridization (FISH) analysis of melanocytic nevi and 
      melanomas: sensitivity, specificity, and lack of association with sentinel node 
      status.
PG  - 434-40
AB  - A 4-color fluorescence in situ hybridization (FISH) assay, using probes to 
      chromosomes 11q, 6p, 6q, and 6 cent, has recently been proposed as an ancillary 
      tool for the diagnosis of melanoma. The authors report herein their experience 
      with this assay. To determine the sensitivity and specificity of the assay for 
      histopathologically unequivocal cases, they analyzed 50 melanocytic nevi, 50 
      primary melanomas, and 15 metastatic melanomas. Of 50 melanocytic nevi, 47 were 
      FISH negative on initial readout (test sensitivity, 94%); 49 were FISH negative 
      after correction for tetraploidy (test specificity, 98%). Of 50 primary 
      melanomas, 41 were FISH positive (test sensitivity, 82%). Of 15 metastatic 
      lesions, 13 were FISH positive (test sensitivity, 85%). Of the 9 FISH-negative 
      melanomas, 6 metastasized. The tumors of the 5 patients who had survived thick 
      primary melanoma for more than 5 years without recurrence were all FISH positive. 
      Half of the patients whose primary melanoma was tested by FISH had undergone 
      sentinel lymph node (SLN) biopsy. When the authors compared the FISH results of 
      those 25 melanomas with the SLN status, no statistically significant correlation 
      was found. These findings document limitations of the current FISH assay. A rare 
      nevus may be FISH positive. Some primary metastasizing melanomas are FISH 
      negative. Even metastatic melanomas can be FISH negative. Awareness of the 
      limitations in test sensitivity and specificity of the FISH assay is important to 
      avoid an erroneous diagnosis by overreliance on cytogenetic findings. Correlation 
      with clinical and histopathological findings is paramount for accurate diagnosis.
FAU - Fang, Yuqiang
AU  - Fang Y
AD  - Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
FAU - Dusza, Stephen
AU  - Dusza S
FAU - Jhanwar, Suresh
AU  - Jhanwar S
FAU - Busam, Klaus J
AU  - Busam KJ
LA  - eng
PT  - Journal Article
DEP - 20120504
PL  - United States
TA  - Int J Surg Pathol
JT  - International journal of surgical pathology
JID - 9314927
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Diagnostic Errors/prevention & control
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Lymph Nodes/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Melanoma/*diagnosis/genetics/surgery
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local
MH  - Nevus, Pigmented/*diagnosis/genetics/surgery
MH  - Predictive Value of Tests
MH  - Sentinel Lymph Node Biopsy
MH  - Skin Neoplasms/*diagnosis/genetics/surgery
MH  - Young Adult
EDAT- 2012/05/09 06:00
MHDA- 2013/02/15 06:00
CRDT- 2012/05/08 06:00
PHST- 2012/05/08 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2013/02/15 06:00 [medline]
AID - 1066896912445923 [pii]
AID - 10.1177/1066896912445923 [doi]
PST - ppublish
SO  - Int J Surg Pathol. 2012 Oct;20(5):434-40. doi: 10.1177/1066896912445923. Epub 
      2012 May 4.