PMID- 22561694
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20131121
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 502
IP  - 2
DP  - 2012 Jul 10
TI  - Prostaglandin J2 series induces the gene expression of monocyte chemoattractant 
      protein-1 during the maturation phase of cultured adipocytes.
PG  - 138-41
LID - 10.1016/j.gene.2012.04.048 [doi]
AB  - Prostaglandin (PG) J(2) series including Delta(12)-PGJ(2) and 
      15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is the dehydration products of 
      PGD(2) that are biosynthesized through the cyclooxygenase (COX) pathway. These 
      prostanoids are active ligands for peroxisome proliferator-activated receptor gamma 
      (PPARgamma), a master regulator of adipogenesis in adipocytes. Here we investigated 
      whether PGJ(2) derivatives can modulate the gene expression of monocyte 
      chemoattractant protein-1 (MCP-1), a pro-inflammatory chemokine, during the 
      maturation phase of adipocytes. Each of selective or nonselective inhibitors for 
      COX isoforms suppressed significantly the accumulation of fats by interfering the 
      induced expression of the PPARgamma gene. Immunological assays of PGJ(2) series 
      revealed higher production of Delta(12)-PGJ(2) than 15d-PGJ(2) by cultured 
      adipocytes, implicating the contribution of endogenous PGJ(2) series to the 
      stimulated adipogenesis. In addition, the increased transcription of MCP-1 was 
      detectable at later maturation phase of adipogenesis, which was prevented by 
      co-incubation with aspirin. Although 15d-PGJ(2) was more potent than 
      Delta(12)-PGJ(2), both PGJ(2) derivatives series had similar effects to rescue 
      dose-dependently the expression of the MCP-1 gene attenuated by aspirin. These 
      findings suggest that the expression of MCP-1 involved in adipocyte inflammation 
      could be positively regulated by the PGJ(2) series during adipogenesis in adipose 
      tissue.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Hossain, Mohammad Salim
AU  - Hossain MS
AD  - Department of Life Science and Biotechnology, Shimane University, Matsue, Shimane 
      690-8504, Japan. pharmasalim@yahoo.com
FAU - Nishimura, Kohji
AU  - Nishimura K
FAU - Jisaka, Mitsuo
AU  - Jisaka M
FAU - Nagaya, Tsutomu
AU  - Nagaya T
FAU - Yokota, Kazushige
AU  - Yokota K
LA  - eng
PT  - Journal Article
DEP - 20120425
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cyclooxygenase Inhibitors)
RN  - 0 (PPAR gamma)
RN  - 60203-57-8 (9-deoxy-delta-9-prostaglandin D2)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/drug effects/metabolism/*physiology
MH  - Adipogenesis/genetics
MH  - Animals
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/*genetics/metabolism
MH  - Cyclooxygenase Inhibitors/pharmacology
MH  - Gene Expression/drug effects
MH  - Mice
MH  - PPAR gamma/biosynthesis/genetics/metabolism
MH  - Prostaglandin D2/*analogs & 
      derivatives/biosynthesis/genetics/metabolism/pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2012/05/09 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/05/08 06:00
PHST- 2011/11/29 00:00 [received]
PHST- 2012/03/05 00:00 [revised]
PHST- 2012/04/18 00:00 [accepted]
PHST- 2012/05/08 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - S0378-1119(12)00474-X [pii]
AID - 10.1016/j.gene.2012.04.048 [doi]
PST - ppublish
SO  - Gene. 2012 Jul 10;502(2):138-41. doi: 10.1016/j.gene.2012.04.048. Epub 2012 Apr 
      25.