PMID- 22563511
OWN - NLM
STAT- MEDLINE
DCOM- 20120830
LR  - 20211021
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Print)
IS  - 1935-2727 (Linking)
VI  - 6
IP  - 5
DP  - 2012
TI  - Cultivation-independent methods reveal differences among bacterial gut microbiota 
      in triatomine vectors of Chagas disease.
PG  - e1631
LID - 10.1371/journal.pntd.0001631 [doi]
LID - e1631
AB  - BACKGROUND: Chagas disease is a trypanosomiasis whose agent is the protozoan 
      parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs 
      known as triatomines. Even though insecticide treatments allow effective control 
      of these bugs in most Latin American countries where Chagas disease is endemic, 
      the disease still affects a large proportion of the population of South America. 
      The features of the disease in humans have been extensively studied, and the 
      genome of the parasite has been sequenced, but no effective drug is yet available 
      to treat Chagas disease. The digestive tract of the insect vectors in which T. 
      cruzi develops has been much less well investigated than blood from its human 
      hosts and constitutes a dynamic environment with very different conditions. Thus, 
      we investigated the composition of the predominant bacterial species of the 
      microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and 
      Dipetalogaster genera. METHODOLOGY/PRINCIPAL FINDINGS: Microbiota of triatomine 
      guts were investigated using cultivation-independent methods, i.e., phylogenetic 
      analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE) and 
      cloned-based sequencing. The Chao index showed that the diversity of bacterial 
      species in triatomine guts is low, comprising fewer than 20 predominant species, 
      and that these species vary between insect species. The analyses showed that 
      Serratia predominates in Rhodnius, Arsenophonus predominates in Triatoma and 
      Panstrongylus, while Candidatus Rohrkolberia predominates in Dipetalogaster. 
      CONCLUSIONS/SIGNIFICANCE: The microbiota of triatomine guts represents one of the 
      factors that may interfere with T. cruzi transmission and virulence in humans. 
      The knowledge of its composition according to insect species is important for 
      designing measures of biological control for T. cruzi. We found that the 
      predominant species of the bacterial microbiota in triatomines form a group of 
      low complexity whose structure differs according to the vector genus.
FAU - da Mota, Fabio Faria
AU  - da Mota FF
AD  - Laboratorio de Biologia Computacional e Sistemas, IOC, FIOCRUZ, Rio de Janeiro, 
      Brazil.
FAU - Marinho, Lourena Pinheiro
AU  - Marinho LP
FAU - Moreira, Carlos Jose de Carvalho
AU  - Moreira CJ
FAU - Lima, Marli Maria
AU  - Lima MM
FAU - Mello, Cicero Brasileiro
AU  - Mello CB
FAU - Garcia, Eloi Souza
AU  - Garcia ES
FAU - Carels, Nicolas
AU  - Carels N
FAU - Azambuja, Patricia
AU  - Azambuja P
LA  - eng
SI  - GENBANK/JQ410794
SI  - GENBANK/JQ410795
SI  - GENBANK/JQ410796
SI  - GENBANK/JQ410797
SI  - GENBANK/JQ410798
SI  - GENBANK/JQ410799
SI  - GENBANK/JQ410800
SI  - GENBANK/JQ410801
SI  - GENBANK/JQ410802
SI  - GENBANK/JQ410803
SI  - GENBANK/JQ410804
SI  - GENBANK/JQ410805
SI  - GENBANK/JQ410806
SI  - GENBANK/JQ410807
SI  - GENBANK/JQ410808
SI  - GENBANK/JQ410809
SI  - GENBANK/JQ410810
SI  - GENBANK/JQ410811
SI  - GENBANK/JQ410812
SI  - GENBANK/JQ410813
SI  - GENBANK/JQ410814
SI  - GENBANK/JQ410815
SI  - GENBANK/JQ410816
SI  - GENBANK/JQ410817
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SI  - GENBANK/JQ410819
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SI  - GENBANK/JQ410821
SI  - GENBANK/JQ410822
SI  - GENBANK/JQ410823
SI  - GENBANK/JQ410824
SI  - GENBANK/JQ410825
SI  - GENBANK/JQ410826
SI  - GENBANK/JQ410827
SI  - GENBANK/JQ410828
SI  - GENBANK/JQ410829
SI  - GENBANK/JQ410830
SI  - GENBANK/JQ410831
SI  - GENBANK/JQ410832
SI  - GENBANK/JQ410833
SI  - GENBANK/JQ410834
SI  - GENBANK/JQ410835
SI  - GENBANK/JQ410836
SI  - GENBANK/JQ410837
SI  - GENBANK/JQ410838
SI  - GENBANK/JQ410839
SI  - GENBANK/JQ410840
SI  - GENBANK/JQ410841
SI  - GENBANK/JQ410842
SI  - GENBANK/JQ410843
SI  - GENBANK/JQ410844
SI  - GENBANK/JQ410845
SI  - GENBANK/JQ410846
SI  - GENBANK/JQ410847
SI  - GENBANK/JQ410848
SI  - GENBANK/JQ410849
SI  - GENBANK/JQ410850
SI  - GENBANK/JQ410851
SI  - GENBANK/JQ410852
SI  - GENBANK/JQ410853
SI  - GENBANK/JQ410854
SI  - GENBANK/JQ410855
SI  - GENBANK/JQ410856
SI  - GENBANK/JQ410857
SI  - GENBANK/JQ410858
SI  - GENBANK/JQ410859
SI  - GENBANK/JQ410860
SI  - GENBANK/JQ410861
SI  - GENBANK/JQ410862
SI  - GENBANK/JQ410863
SI  - GENBANK/JQ410864
SI  - GENBANK/JQ410865
SI  - GENBANK/JQ410866
SI  - GENBANK/JQ410867
SI  - GENBANK/JQ410868
SI  - GENBANK/JQ410869
SI  - GENBANK/JQ410870
SI  - GENBANK/JQ410871
SI  - GENBANK/JQ410872
SI  - GENBANK/JQ410873
SI  - GENBANK/JQ410874
SI  - GENBANK/JQ410875
SI  - GENBANK/JQ410876
SI  - GENBANK/JQ410877
SI  - GENBANK/JQ410878
SI  - GENBANK/JQ410879
SI  - GENBANK/JQ410880
SI  - GENBANK/JQ410881
SI  - GENBANK/JQ410882
SI  - GENBANK/JQ410883
SI  - GENBANK/JQ410884
SI  - GENBANK/JQ410885
SI  - GENBANK/JQ410886
SI  - GENBANK/JQ410887
SI  - GENBANK/JQ410888
SI  - GENBANK/JQ410889
SI  - GENBANK/JQ410890
SI  - GENBANK/JQ410891
SI  - GENBANK/JQ410892
SI  - GENBANK/JQ410893
SI  - GENBANK/JQ410894
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120501
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/*genetics
MH  - *Biodiversity
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - *Disease Vectors
MH  - Female
MH  - Gastrointestinal Tract/microbiology
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - South America
MH  - Triatominae/*microbiology
PMC - PMC3341335
COIS- The authors have declared that no competing interests exist.
EDAT- 2012/05/09 06:00
MHDA- 2012/08/31 06:00
PMCR- 2012/05/01
CRDT- 2012/05/08 06:00
PHST- 2011/10/05 00:00 [received]
PHST- 2012/03/17 00:00 [accepted]
PHST- 2012/05/08 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/08/31 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - PNTD-D-11-00971 [pii]
AID - 10.1371/journal.pntd.0001631 [doi]
PST - ppublish
SO  - PLoS Negl Trop Dis. 2012;6(5):e1631. doi: 10.1371/journal.pntd.0001631. Epub 2012 
      May 1.