PMID- 22563925
OWN - NLM
STAT- MEDLINE
DCOM- 20130429
LR  - 20131121
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 80
IP  - 2
DP  - 2012 Aug
TI  - In vitro up-regulation of HLA-G using dexamethasone and hydrocortisone in 
      first-trimester trophoblast cells of women experiencing recurrent miscarriage.
PG  - 126-35
LID - 10.1111/j.1399-0039.2012.01884.x [doi]
AB  - The trophoblast cells at the maternal-fetal interface express an unusual 
      combination of human leukocyte antigen (HLA)-C, HLA-E and HLA-G. Altered 
      expression of HLA-G on the extravillous cytotrophoblast has been implicated in 
      the etiology of recurrent miscarriages (RMs). We have assessed HLA-G expression 
      in extravillous cytotrophoblast in cell cultures prepared from RM patients and 
      compared with those of first-trimester voluntarily terminated normal pregnancies 
      (control). Glucocorticoids, dexamethasone and hydrocortisone were examined for 
      their role in modulation of the HLA-G expression. HLA-G promoter and 3'UTR 
      variants were investigated for their effect on the transcription of HLA-G. 
      Cultured cytotrophoblast cells from the first-trimester RM patients were treated 
      with dexamethasone and hydrocortisone (dose concentration 0-1000 ng/ml). HLA-G 
      gene transcription was determined by semiquantitative and quantitative real-time 
      polymerase chain reaction (RT-PCR), while protein expression was determined by a 
      specific enzyme-linked immunosorbent assay (ELISA), flow cytometry and western 
      blot analyses. HLA-G polymorphisms were detected by PCR and/or sequence-based 
      typing. Low level of HLA-G was observed in untreated trophoblast cells obtained 
      from RM patients as compared with controls. Upon treatment with glucocorticoids, 
      the expression of HLA-G in these cells was up-regulated in a dose-dependent 
      manner (P < 0.05), with no change in cellular proliferation and viability. There 
      was no significant association between HLA-G polymorphism in RM patients and 
      controls. HLA-G is minimally expressed in cultured trophoblast cells of RM 
      patients. It can be up-regulated upon exposure with both dexamethasone and 
      hydrocortisone. Glucocorticoids have the potential to modulate HLA-G expression 
      in vitro, and can be further examined for their therapeutic applicability in RM.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Akhter, A
AU  - Akhter A
AD  - Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical 
      Sciences, Lucknow, Uttar Pradesh, India.
FAU - Faridi, R M
AU  - Faridi RM
FAU - Das, V
AU  - Das V
FAU - Pandey, A
AU  - Pandey A
FAU - Naik, S
AU  - Naik S
FAU - Agrawal, S
AU  - Agrawal S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120504
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA-G Antigens)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Abortion, Habitual/*genetics/immunology
MH  - Adult
MH  - Dexamethasone/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gene Expression/drug effects
MH  - HLA-G Antigens/*genetics/immunology
MH  - Humans
MH  - Hydrocortisone/*pharmacology
MH  - Polymorphism, Genetic
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Primary Cell Culture
MH  - Promoter Regions, Genetic
MH  - Transcription, Genetic
MH  - Trophoblasts/cytology/*drug effects/metabolism
MH  - Up-Regulation/drug effects/genetics/immunology
EDAT- 2012/05/09 06:00
MHDA- 2013/04/30 06:00
CRDT- 2012/05/09 06:00
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2013/04/30 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01884.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Aug;80(2):126-35. doi: 10.1111/j.1399-0039.2012.01884.x. 
      Epub 2012 May 4.