PMID- 22564420
OWN - NLM
STAT- MEDLINE
DCOM- 20120808
LR  - 20211021
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 429
IP  - 2
DP  - 2012 Aug 1
TI  - Autophagy sustains the replication of porcine reproductive and respiratory virus 
      in host cells.
PG  - 136-47
LID - 10.1016/j.virol.2012.03.022 [doi]
AB  - In this study, we confirmed the autophagy induced by porcine reproductive and 
      respiratory syndrome virus (PRRSV) in permissive cells and investigated the role 
      of autophagy in the replication of PRRSV. We first demonstrated that PRRSV 
      infection significantly results in the increased double-membrane vesicles, the 
      accumulation of LC3 fluorescence puncta, and the raised ratio of LC3-II/beta-actin, 
      in MARC-145 cells. Then we discovered that induction of autophagy by rapamycin 
      significantly enhances the viral titers of PRRSV, while inhibition of autophagy 
      by 3-MA and silencing of LC3 gene by siRNA reduces the yield of PRRSV. The 
      results showed functional autolysosomes can be formed after PRRSV infection and 
      the autophagosome-lysosome-fusion inhibitor decreases the virus titers. We also 
      examined the induction of autophagy by PRRSV infection in pulmonary alveolar 
      macrophages. These findings indicate that autophagy induced by PRRSV infection 
      plays a role in sustaining the replication of PRRSV in host cells.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Liu, Qinghao
AU  - Liu Q
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agribiotechnology, 
      China Agricultural University, Beijing, People's Republic of China.
FAU - Qin, Yixian
AU  - Qin Y
FAU - Zhou, Lei
AU  - Zhou L
FAU - Kou, Qiuwen
AU  - Kou Q
FAU - Guo, Xin
AU  - Guo X
FAU - Ge, Xinna
AU  - Ge X
FAU - Yang, Hanchun
AU  - Yang H
FAU - Hu, Hongbo
AU  - Hu H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120506
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - *Autophagy
MH  - Cells, Cultured
MH  - Haplorhini
MH  - *Host-Pathogen Interactions
MH  - Macrophages, Alveolar/virology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Sirolimus/metabolism
MH  - Swine
MH  - Viral Load
MH  - *Virus Replication
PMC - PMC7111961
EDAT- 2012/05/09 06:00
MHDA- 2012/08/09 06:00
PMCR- 2012/05/05
CRDT- 2012/05/09 06:00
PHST- 2011/12/03 00:00 [received]
PHST- 2012/01/18 00:00 [revised]
PHST- 2012/03/30 00:00 [accepted]
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/08/09 06:00 [medline]
PHST- 2012/05/05 00:00 [pmc-release]
AID - S0042-6822(12)00179-1 [pii]
AID - 10.1016/j.virol.2012.03.022 [doi]
PST - ppublish
SO  - Virology. 2012 Aug 1;429(2):136-47. doi: 10.1016/j.virol.2012.03.022. Epub 2012 
      May 6.