PMID- 22564536
OWN - NLM
STAT- MEDLINE
DCOM- 20121009
LR  - 20161125
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 262
IP  - 2
DP  - 2012 Jul 15
TI  - Inhibitory effects of diallyl disulfide on the production of inflammatory 
      mediators and cytokines in lipopolysaccharide-activated BV2 microglia.
PG  - 177-84
LID - 10.1016/j.taap.2012.04.034 [doi]
AB  - Diallyl disulfide (DADS), a main organosulfur component responsible for the 
      diverse biological effects of garlic, displays a wide variety of internal 
      biological activities. However, the cellular and molecular mechanisms underlying 
      DADS' anti-inflammatory activity remain poorly understood. In this study, 
      therefore, the anti-inflammatory effects of DADS were studied to investigate its 
      potential therapeutic effects in lipopolysaccharide (LPS)-stimulated BV2 
      microglia. We found that pretreatment with DADS prior to treatment with LPS 
      significantly inhibited excessive production of nitric oxide (NO) and 
      prostaglandin E(2) (PGE(2)) in a dose-dependent manner. The inhibition was associated 
      with down-regulation of inducible nitric oxide synthase (iNOS) and 
      cyclooxygenase-2 (COX-2) expression. DADS also attenuated the production of 
      pro-inflammatory cytokines and chemokines, including interleukin-1beta (IL-1beta), 
      tumor necrosis factor (TNF)-alpha, and monocyte chemoattractant protein-1 (MCP-1) by 
      suppressing the expression of mRNAs for these proteins. The mechanism underlying 
      this protective effect might be related to the inhibition of nuclear 
      factor-kappaB, Akt and mitogen-activated protein kinase signaling pathway 
      activation in LPS-stimulated microglial cells. These findings indicated that DADS 
      is potentially a novel therapeutic candidate for the treatment of various 
      neurodegenerative diseases.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Park, Hye Young
AU  - Park HY
AD  - Department of Biochemistry, Dongeui University College of Oriental Medicine, 
      Busan 614-714, Republic of Korea.
FAU - Kim, Nam Deuk
AU  - Kim ND
FAU - Kim, Gi-Young
AU  - Kim GY
FAU - Hwang, Hye Jin
AU  - Hwang HJ
FAU - Kim, Byung-Woo
AU  - Kim BW
FAU - Kim, Wun Jae
AU  - Kim WJ
FAU - Choi, Yung Hyun
AU  - Choi YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120504
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Allyl Compounds)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Disulfides)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 5HI47O6OA7 (diallyl disulfide)
RN  - 63231-63-0 (RNA)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Allyl Compounds/*pharmacology
MH  - Blotting, Western
MH  - Cell Survival/drug effects
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - Cyclooxygenase 2/*biosynthesis/genetics
MH  - Dinoprostone/*metabolism
MH  - Disulfides/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-1beta/biosynthesis/genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Microglia/*drug effects/enzymology/immunology
MH  - Neurodegenerative Diseases/enzymology/*immunology
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase Type II/*biosynthesis/genetics
MH  - RNA/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 2012/05/09 06:00
MHDA- 2012/10/10 06:00
CRDT- 2012/05/09 06:00
PHST- 2011/12/23 00:00 [received]
PHST- 2012/04/22 00:00 [revised]
PHST- 2012/04/26 00:00 [accepted]
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/10/10 06:00 [medline]
AID - S0041-008X(12)00185-8 [pii]
AID - 10.1016/j.taap.2012.04.034 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2012 Jul 15;262(2):177-84. doi: 
      10.1016/j.taap.2012.04.034. Epub 2012 May 4.