PMID- 22564604
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20131121
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 44
IP  - 4
DP  - 2012 May
TI  - Effects of cyclosporine on oxidative stress in human bronchial epithelial cells.
PG  - 988-90
LID - 10.1016/j.transproceed.2012.01.091 [doi]
AB  - OBJECTIVES: Some of the airway complications relate to the use of cyclosporine 
      (CsA), a potent agent widely used after organ transplantations. Several recent 
      studies have demonstrated CsA treatment to induce reactive oxygen species (ROS). 
      The present study was undertaken to investigate effects of CsA on production of 
      ROS and antoxidant defense of airway cells using the human bronchial epithelial 
      cell line BEAS-2B. METHODS: We measured biological antioxidant potential (BAP), 
      as well as ROS and malondialdehyde levels in BEAS-2B cells after CsA treatment, 
      using Free Radical Analytical System 4 kits (Diacron, Grosseto, Italy). ROS 
      production was expressed as Carr Units as established by the manufacturer and BAP 
      as mumol/2 x 10(5) cells; malondialdehyde, by the thiobarbituric acid assay. 
      RESULTS: ROS production was increased in the BEAS-2B cells after CsA treatment: 
      73.5 at 0 (controls); 82.5 at 10; 84.0 at 30; 86.0 at 50; and 93.0 Carr Unit/2 x 
      10(5) cells at 100 mug/mL of CsA. The levels of BAP were 1821 at 0 (controls), 
      1698 at 10; 1653 at 30; 1366 at 50 mug/mL; and 1391 at 100 mug/mL. The levels of 
      malondialdehyde were increased: 3.8 at 0 (controls); 3.4 at 10; 4.4 at 30; 4.2 at 
      50: and 5.0 nmol/10(6) cells at 100 mug/mL. CONCLUSIONS: Increased production of 
      ROS and decreased BAP by CsA in BEAS-2B cells may increase malondialdehyde levels 
      by radical-induced damage.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Jeon, D S
AU  - Jeon DS
AD  - Institute for Medical Science, Keimyung University, Daegu, Korea.
FAU - Ha, E Y
AU  - Ha EY
FAU - Mun, K C
AU  - Mun KC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Antioxidants)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Antioxidants/metabolism
MH  - Bronchi/cytology/*drug effects/metabolism
MH  - Cell Line
MH  - Cyclosporine/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Immunosuppressive Agents/*pharmacology
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2012/05/09 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/05/09 06:00
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - S0041-1345(12)00126-1 [pii]
AID - 10.1016/j.transproceed.2012.01.091 [doi]
PST - ppublish
SO  - Transplant Proc. 2012 May;44(4):988-90. doi: 10.1016/j.transproceed.2012.01.091.