PMID- 22564634
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20220321
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 44
IP  - 4
DP  - 2012 May
TI  - Hybrid cellular spheroids from hepatocellular carcinoma and insulin-secreting 
      cell lines.
PG  - 1095-8
LID - 10.1016/j.transproceed.2012.02.016 [doi]
AB  - During islet transplantation into the portal vein of the liver, the islet cells 
      are expected to have complex interactions with hepatocytes. However, the 
      mechanism underlying this interaction is not yet understood. Hence, we developed 
      cellular complexes containing a mixture of human hepatocellular carcinoma cell 
      line (Hep-G2) and rat insulin-secreting cell line (RIN-5F) by using a co-culture 
      model and studied the function and morphology of the resultant hybrid cellular 
      spheroids (HCSs). The RIN-5F and Hep-G2 cells were suspension cultured and, 
      within 5 days of culture, the two types of cells aggregated to yield spheroids. 
      The functionality of the thus formed HCSs was evaluated by measuring the levels 
      of insulin and albumin in the culture supernatant. The HCSs retained their 
      insulin- and albumin-secreting ability and their morphology, as revealed by 
      immunohistological staining. The insulin and albumin levels secreted by the HCSs 
      were considerably higher than those secreted by spheroids of single-cell origin. 
      Generally, obtaining complexes from more than two types of cells is difficult. 
      However, we were able to generate HCSs. We believe that this culture method could 
      have various applications such as studying the in vitro cell-cell interactions 
      and developing new cell transplantation models.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Kim, J Y
AU  - Kim JY
AD  - The Research Institute for Transplantation, Yonsei University Health System, 
      Seoul, Korea.
FAU - Kim, H W
AU  - Kim HW
FAU - Bae, S J
AU  - Bae SJ
FAU - Joo, D J
AU  - Joo DJ
FAU - Huh, K H
AU  - Huh KH
FAU - Fang, Y H
AU  - Fang YH
FAU - Cho, Y
AU  - Cho Y
FAU - Jeong, J H
AU  - Jeong JH
FAU - Kim, Y S
AU  - Kim YS
FAU - Lee, J I
AU  - Lee JI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Albumins)
RN  - 0 (Insulin)
SB  - IM
MH  - Albumins/metabolism
MH  - Animals
MH  - Apoptosis
MH  - Carcinoma, Hepatocellular/*metabolism/pathology
MH  - *Cell Communication
MH  - Coculture Techniques
MH  - Hep G2 Cells
MH  - Humans
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Insulin-Secreting Cells/*metabolism/pathology
MH  - Liver Neoplasms/*metabolism/pathology
MH  - Rats
MH  - Spheroids, Cellular
MH  - Time Factors
EDAT- 2012/05/09 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/05/09 06:00
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - S0041-1345(12)00204-7 [pii]
AID - 10.1016/j.transproceed.2012.02.016 [doi]
PST - ppublish
SO  - Transplant Proc. 2012 May;44(4):1095-8. doi: 10.1016/j.transproceed.2012.02.016.