PMID- 22565217
OWN - NLM
STAT- MEDLINE
DCOM- 20121106
LR  - 20131121
IS  - 1095-6867 (Electronic)
IS  - 0018-506X (Linking)
VI  - 62
IP  - 1
DP  - 2012 Jun
TI  - Sexual responses of the male rat medial preoptic area and medial amygdala to 
      estrogen I: site specific suppression of estrogen receptor alpha.
PG  - 50-7
LID - 10.1016/j.yhbeh.2012.04.018 [doi]
AB  - Male rat copulation is mediated by estrogen-sensitive neurons in the medial 
      preoptic area (MPO) and medial amygdala (MEA); however, the mechanisms through 
      which estradiol (E(2)) acts are not fully understood. We hypothesized that E(2) 
      acts through estrogen receptor alpha (ERalpha) in the MPO and MEA to promote male mating 
      behavior. Antisense oligodeoxynucleotides (AS-ODN) complementary to ERalpha mRNA were 
      bilaterally infused via minipumps into either brain area to block the synthesis 
      of ERalpha, which we predicted would reduce mating. Western blot analysis and 
      immunocytochemistry revealed a knockdown of ERalpha expression in each brain region; 
      however, compared to saline controls, males receiving AS-ODN to the MPO showed 
      significant reductions in all components of mating, whereas males receiving 
      AS-ODN to the MEA continued to mate normally. These results suggest that E(2) 
      acts differently in these brain regions to promote the expression of male rat 
      sexual behavior and that ERalpha in the MPO, but not in the MEA, promotes mating.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Paisley, Jacquelyn C
AU  - Paisley JC
AD  - Department of Biology, Georgia State University, Atlanta, GA 30302-4010, USA.
FAU - Huddleston, Gloria G
AU  - Huddleston GG
FAU - Carruth, Laura L
AU  - Carruth LL
FAU - Petrulis, Aras
AU  - Petrulis A
FAU - Grober, Matthew S
AU  - Grober MS
FAU - Clancy, Andrew N
AU  - Clancy AN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120504
PL  - United States
TA  - Horm Behav
JT  - Hormones and behavior
JID - 0217764
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Amygdala/drug effects/*physiology
MH  - Animals
MH  - Copulation/drug effects/*physiology
MH  - Estradiol/*metabolism/pharmacology
MH  - Estrogen Receptor alpha/antagonists & inhibitors/genetics/*metabolism
MH  - Female
MH  - Male
MH  - Oligodeoxyribonucleotides, Antisense/genetics
MH  - Preoptic Area/drug effects/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley/genetics/*physiology
EDAT- 2012/05/09 06:00
MHDA- 2012/11/07 06:00
CRDT- 2012/05/09 06:00
PHST- 2012/01/11 00:00 [received]
PHST- 2012/03/31 00:00 [revised]
PHST- 2012/04/28 00:00 [accepted]
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/11/07 06:00 [medline]
AID - S0018-506X(12)00140-7 [pii]
AID - 10.1016/j.yhbeh.2012.04.018 [doi]
PST - ppublish
SO  - Horm Behav. 2012 Jun;62(1):50-7. doi: 10.1016/j.yhbeh.2012.04.018. Epub 2012 May 
      4.