PMID- 22565262
OWN - NLM
STAT- MEDLINE
DCOM- 20120614
LR  - 20161125
IS  - 0047-2425 (Print)
IS  - 0047-2425 (Linking)
VI  - 41
IP  - 3
DP  - 2012 May-Jun
TI  - Polyphenol oxidase activity in subcellular fractions of tall fescue contaminated 
      by polycyclic aromatic hydrocarbons.
PG  - 807-13
LID - 10.2134/jeq2011.0461 [doi]
AB  - Understanding enzyme responses to contamination with persistent organic 
      pollutants (POPs) is a key step in the elucidation of POP metabolic mechanisms in 
      plants. However, there is little information available on enzyme activity in 
      subcellular fractions of POP-contaminated plants. To our knowledge, this is the 
      first study to investigate the activities of polyphenol oxidase (PPO) in cell 
      fractions of plants under contamination stress from polycyclic aromatic 
      hydrocarbons (PAHs) using a greenhouse batch technique. Three parameters, 
      E(cell), E(cell-n), and P(cell), denoting the amount of PPO activity, cell 
      fraction content-normalized PPO activity, and proportion of PPO activity in each 
      cell fraction, respectively, were used in this study. Contamination with 
      phenanthrene, as a representative PAH, at a relatively high level (>0.23 mg L(-)(1)) 
      in culture solution generally stimulated PPO activity in tall fescue (Festuca 
      arundinacea Schreb.) roots and shoots and their cellular fractions. The amount 
      and distribution proportion of PPO activity in each cell fraction of 
      phenanthrene-contaminated roots and shoots were (in descending order): cell 
      solution > > cell wall > cell organelles. Cell solution was the dominant storage 
      domain of PPO activity and contributed 84.0 and 82.8% of PPO activity in roots 
      and shoots, respectively. The cell wall had the highest density of PPO activity 
      in roots and shoots, based on the highest cell fraction content normalized PPO 
      activity in this cell fraction. Our results provide new information on enzyme 
      responses in plant intracellular fractions to xenobiotic POPs and fundamental 
      information on within-plant POP metabolic mechanisms.
CI  - Copyright (c) by the American Society of Agronomy, Crop Science Society of America, 
      and Soil Science Society of America, Inc.
FAU - Ling, Wanting
AU  - Ling W
AD  - Institute of Organic Contaminant Control and Soil Remediation, College of 
      Resource and Environmental Sciences, Nanjing Agricultural Univ., P.R. China.
FAU - Lu, Xiaodan
AU  - Lu X
FAU - Gao, Yanzheng
AU  - Gao Y
FAU - Liu, Juan
AU  - Liu J
FAU - Sun, Yandi
AU  - Sun Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Environ Qual
JT  - Journal of environmental quality
JID - 0330666
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - EC 1.10.3.1 (Catechol Oxidase)
SB  - IM
MH  - Catechol Oxidase/*metabolism
MH  - Environmental Pollutants/chemistry/metabolism/*toxicity
MH  - Poaceae/*enzymology
MH  - Polycyclic Aromatic Hydrocarbons/chemistry/metabolism/*toxicity
EDAT- 2012/05/09 06:00
MHDA- 2012/06/15 06:00
CRDT- 2012/05/09 06:00
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/06/15 06:00 [medline]
AID - 10.2134/jeq2011.0461 [doi]
PST - ppublish
SO  - J Environ Qual. 2012 May-Jun;41(3):807-13. doi: 10.2134/jeq2011.0461.