PMID- 22566199
OWN - NLM
STAT- MEDLINE
DCOM- 20121016
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 403
IP  - 9
DP  - 2012 Jul
TI  - Evaluation of hair roots for detection of methamphetamine and 
      3,4-methylenedioxymethamphetamine abuse by use of an HPLC-chemiluminescence 
      method.
PG  - 2569-76
LID - 10.1007/s00216-012-6054-z [doi]
AB  - We describe the use of hair roots as a matrix for detection of methamphetamine 
      (MP) and 3,4-methylenedioxymethamphetamine (MDMA) abuse. The concentration of 
      drugs was determined in rat hair roots, hair shafts, and plasma after a single 
      administration of MP or MDMA, by use of an HPLC-peroxyoxalate chemiluminescence 
      (PO-CL) method involving column switching. Plasma and hair roots and shafts were 
      collected from male Wistar rats before and after administration of MP (10 mg 
      kg(-1), i.p.). In addition, the roots and shafts of pigmented and non-pigmented 
      hair of male Lister hooded rats were collected after administration of MDMA (10 
      mg kg(-1), i.p.). The concentrations of MP and MDMA in plasma and hair were 
      determined by use of the HPLC-PO-CL method, with satisfactory sensitivity and 
      reproducibility. The concentration of MP in hair roots 1-14 days after 
      administration ranged from 0.038 to 0.115 ng mg(-1) (n = 3). By use of the 
      HPLC-PO-CL method, MP could be detected in hair roots for longer (up to 14 days) 
      than it could be detected in conventional biological specimens, for example 
      plasma (~1 day), and MDMA was detected in hair roots from 1 to 10 days after 
      administration. The AUC(1-10) (ng day mg(-1)) for MDMA in roots of non-pigmented 
      and pigmented hair was comparable (4.93 +/- 2.09 vs. 6.67 +/- 1.28, n = 3), whereas 
      AUC(1-14) for hair shafts differed significantly (1.86 +/- 0.93 vs. 4.58 +/- 0.63, P 
      < 0.05, n = 3). The window for detecting MP (or MDMA) in hair roots under our 
      conditions was 1-14 (or 1-10) days.
FAU - Wada, Mitsuhiro
AU  - Wada M
AD  - Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. 
      m-wada@nagasaki-u.ac.jp
FAU - Ochi, Yuko
AU  - Ochi Y
FAU - Nogami, Kumi
AU  - Nogami K
FAU - Ikeda, Rie
AU  - Ikeda R
FAU - Kuroda, Naotaka
AU  - Kuroda N
FAU - Nakashima, Kenichiro
AU  - Nakashima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20120509
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Central Nervous System Stimulants)
RN  - 44RAL3456C (Methamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Central Nervous System Stimulants/administration & dosage/*analysis/blood
MH  - Chromatography, High Pressure Liquid/instrumentation/methods
MH  - Equipment Design
MH  - Hair/*chemistry
MH  - Luminescent Measurements/instrumentation/*methods
MH  - Male
MH  - Methamphetamine/administration & dosage/*analysis/blood
MH  - N-Methyl-3,4-methylenedioxyamphetamine/administration & dosage/*analysis/blood
MH  - Rats
MH  - Rats, Wistar
MH  - Sensitivity and Specificity
MH  - Substance Abuse Detection/instrumentation/*methods
EDAT- 2012/05/09 06:00
MHDA- 2012/10/17 06:00
CRDT- 2012/05/09 06:00
PHST- 2011/12/26 00:00 [received]
PHST- 2012/04/15 00:00 [accepted]
PHST- 2012/03/23 00:00 [revised]
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/10/17 06:00 [medline]
AID - 10.1007/s00216-012-6054-z [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2012 Jul;403(9):2569-76. doi: 10.1007/s00216-012-6054-z. Epub 
      2012 May 9.