PMID- 22567049
OWN - NLM
STAT- MEDLINE
DCOM- 20120830
LR  - 20211021
IS  - 1844-3117 (Electronic)
IS  - 1844-122X (Print)
IS  - 1844-122X (Linking)
VI  - 4
IP  - 3
DP  - 2011 Aug 15
TI  - Cytokine gene polymorphisms support diagnostic monitoring of Romanian multiple 
      myeloma patients.
PG  - 264-8
AB  - INTRODUCTION: Cytokines and their receptor genes are very polymorphic. SNPs in 
      the promotor region of the gene may influence the rate of cytokine secretion and 
      may affect the biological activity of the encoded cytokine. A number of cytokines 
      and cytokine receptors have been directly linked to the development of human 
      cancers. The aim of our study was to determine the cytokine gene polymorphism in 
      Romanian multiple myeloma patients. MATERIAL AND METHODS: Cytokine genotyping was 
      performed in 80 patients and 100 healthy blood donors using molecular biology 
      methods (SSP-Invitrogen, USA). RESULTS: Analyzing each polymorphic site, there 
      was an increased frequency of the following genotypes in patients compared to 
      control group: Interleukin-1beta (IL-1beta) pos.+3962 TT, IL-12 pos.-1188 CC, 
      gamma-Interferon (gamma-IFN) pos.+874 AA, Transforming Growth Factor- beta1 (TGF- beta1) 
      codon10 TT, IL-2 pos.-330 TG and pos.+166 TT, Interleukin-4Receptor alpha 
      (IL-4Ralpha) pos.-33 TC, IL-10 pos.-1082 GG and pos.-592 CC, IL-6 pos.-174 GG. It 
      should be noted that almost one third of multiple myeloma patients had IL-6 
      pos.-174 GG genotype and 62% IL-10 GCC haplotype. These identified haplotypes are 
      high interleukins producer, and this fact was confirmed by serum IL-6 and IL-10 
      levels performed by ELISA and enhanced chemiluminiscence methods. CONCLUSION: 
      These markers could be successfully used, together with other specific clinical 
      and biological parameters, as reliable individualized prognostic factors in 
      multiple myeloma patients.
FAU - Banu, C
AU  - Banu C
AD  - National Institute for Medical Assessment and Work Capacity Rehabilitation, 
      Bucharest, Romania. krsbanu@yahoo.com
FAU - Moise, A
AU  - Moise A
FAU - Arion, C V
AU  - Arion CV
FAU - Coriu, D
AU  - Coriu D
FAU - Tanase, A
AU  - Tanase A
FAU - Constantinescu, I
AU  - Constantinescu I
LA  - eng
PT  - Journal Article
DEP - 20110825
PL  - Romania
TA  - J Med Life
JT  - Journal of medicine and life
JID - 101477617
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Cytokines/blood/*genetics
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Monitoring, Immunologic
MH  - Multiple Myeloma/classification/*diagnosis/*genetics/immunology
MH  - Neoplasm Staging
MH  - *Polymorphism, Genetic
MH  - Romania
PMC - PMC3168821
OTO - NOTNLM
OT  - cytokine gene polymorphism
OT  - interleukins
OT  - multiple myeloma
EDAT- 2012/05/09 06:00
MHDA- 2012/08/31 06:00
PMCR- 2011/10/15
CRDT- 2012/05/09 06:00
PHST- 2011/05/07 00:00 [received]
PHST- 2011/07/04 00:00 [accepted]
PHST- 2012/05/09 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/08/31 06:00 [medline]
PHST- 2011/10/15 00:00 [pmc-release]
AID - JMedLife-04-264 [pii]
PST - ppublish
SO  - J Med Life. 2011 Aug 15;4(3):264-8. Epub 2011 Aug 25.