PMID- 22568335
OWN - NLM
STAT- MEDLINE
DCOM- 20121220
LR  - 20181201
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 26
IP  - 4
DP  - 2012 Apr
TI  - [Study on bone marrow mesenchymal stem cells derived osteoblasts and endothelial 
      cells compound with chitosan/hydroxyapatite scaffold to construct vascularized 
      tissue engineered bone].
PG  - 489-94
AB  - OBJECTIVE: To explore the osteogenesis and angiogenesis effect of bone marrow 
      mesenchymal stem cells (BMSCs) derived osteoblasts and endothelial cells compound 
      with chitosan/hydroxyapatite (CS/HA) scaffold in repairing radial defect in rats. 
      METHODS: The BMSCs were isolated from Sprague Dawley rats and the 3rd generation 
      of BMSCs were induced into osteoblasts and endothelial cells. The endothelial 
      cells, osteoblasts, and mixed osteoblasts and endothelial cells (1:1) were 
      compound with CS/HA scaffold in groups A, B, and C respectively to prepare the 
      cell-scaffold composites. The cell proliferation was detected by MTT. The rat 
      radial segmental defect model was made and the 3 cell-scaffolds were implanted, 
      respectively. At 4, 8, and 12 weeks after transplantation, the graft was 
      harvested to perform HE staining and CD34 immunohistochemistry staining. The mRNA 
      expressions of osteopontin (OPN) and osteoprotegerin (OPG) were detected by 
      RT-PCR. RESULTS: Alkaline phosphatase staining of osteoblasts showed that there 
      were blue grains in cytoplasm at 7 days after osteogenic induction and the nuclei 
      were stained red. CD34 immunocytochemical staining of the endothelial cells 
      showed that there were brown grains in the cytoplasm at 14 days after 
      angiogenesis induction. MTT test showed that the proliferation level of the cells 
      in 3 groups increased with the time. HE staining showed that no obvious osteoid 
      formation, denser microvessel, and more fibrous tissue were seen at 12 weeks in 
      group A; homogeneous osteoid which distributed with cord or island, and many 
      osteoblast-like cells were seen in groups B and C. The microvessel density was 
      significantly higher in groups A and C than group B at 3 time points (P < 0.05), 
      and in group A than in group C at 12 weeks (P < 0.05). The OPN and OPG mRNA 
      expressions of group A were significantly lower than those of groups B and C at 3 
      time points (P < 0.05). In groups B and C, the OPN mRNA expressions reached peak 
      at 8 and 12 weeks, respectively, and OPG mRNA expressions reached peak at 4 
      weeks. CONCLUSION: BMSCs derived osteoblasts and endothelial cells (1:1) compound 
      with CS/HA porous scaffold can promote bone formation and vascularization in bone 
      defect and accelerate the healing of bone defect.
FAU - Hao, Zengtao
AU  - Hao Z
AD  - Department of Trauma and Orthopaedics, Nanfang Hospital, Southern Medical 
      University, Guangzhou Guangdong, 510515, P.R.China.
FAU - Feng, Wei
AU  - Feng W
FAU - Hao, Tini
AU  - Hao T
FAU - Yu, Bin
AU  - Yu B
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Biocompatible Materials)
RN  - 9012-76-4 (Chitosan)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Bone Marrow Cells/cytology
MH  - Cells, Cultured
MH  - Chitosan/therapeutic use
MH  - Coculture Techniques
MH  - Disease Models, Animal
MH  - Durapatite/therapeutic use
MH  - Endothelial Cells/*cytology
MH  - Male
MH  - Mesenchymal Stem Cells/cytology
MH  - Neovascularization, Physiologic
MH  - Osteoblasts/*cytology
MH  - *Osteogenesis
MH  - Radius/injuries/*surgery
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Engineering/methods
MH  - *Tissue Scaffolds
EDAT- 2012/05/10 06:00
MHDA- 2012/12/21 06:00
CRDT- 2012/05/10 06:00
PHST- 2012/05/10 06:00 [entrez]
PHST- 2012/05/10 06:00 [pubmed]
PHST- 2012/12/21 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Apr;26(4):489-94.