PMID- 22569742
OWN - NLM
STAT- MEDLINE
DCOM- 20130913
LR  - 20211021
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 32
IP  - 8
DP  - 2012 Nov
TI  - Neural differentiation of rat adipose-derived stem cells in vitro.
PG  - 1255-63
LID - 10.1007/s10571-012-9850-2 [doi]
AB  - It is reported that adipose-derived stem cells (ADSCs) had multilineage 
      differentiation potential, and could differentiate into neuron-like cells induced 
      by special induction media, which may provide a new idea for restoration of 
      erectile dysfunction (ED) after cavernous nerve injury. The aim of this research 
      was to explore the neuronal differentiation potential of ADSCs in vitro. ADSCs 
      isolated from inguinal adipose tissue of rat were characterized by flow 
      cytometry, and results showed that ADSCs were positive for mesenchymal stem cell 
      markers CD90 and CD44, but negative for hematopoietic stem cell markers. ADSCs 
      maintained self-renewing capacity and could differentiate into adipocytes and 
      neurocytes under special culture condition. In this research, two methods were 
      used to induce ADSCs. In method 1, ADSCs were treated with the preinduction 
      medium including epithelium growth factor, basic fibroblast growth factor, and 
      brain derived neurotrophic factor (BDNF) for 3 days, then with the neurogenic 
      induction medium containing isobutylmethylxanthine, indomethacin, and insulin. 
      While in method 2, BDNF was not used to treat ADSCs. After induction, neuronal 
      differentiation of ADSCs was evaluated. Neuronal markers, glial fibrillary acidic 
      protein (GFAP), and beta-tubulin III (Tuj-1) were detected by immunofluorescence and 
      Western Blot analyses. The expressions of GFAP and Tuj-1 in method 1 were 
      obviously higher then those in method 2. In addition, the positive rate of the 
      neuron-like cells was higher in method 1. It suggested that ADSCs are able to 
      differentiate into neural-like cells in vitro, and the administration of BDNF in 
      the preinduction medium may provide a new way to modify the culture method for 
      getting more neuron-like cells in vitro.
FAU - Ying, Chengcheng
AU  - Ying C
AD  - Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China.
FAU - Hu, Wanli
AU  - Hu W
FAU - Cheng, Bei
AU  - Cheng B
FAU - Zheng, Xinmin
AU  - Zheng X
FAU - Li, Shiwen
AU  - Li S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120509
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
SB  - IM
MH  - Adipose Tissue/*cytology/*physiology
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - Female
MH  - Male
MH  - Neurons/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/*physiology
EDAT- 2012/05/10 06:00
MHDA- 2013/09/14 06:00
CRDT- 2012/05/10 06:00
PHST- 2011/11/29 00:00 [received]
PHST- 2012/04/17 00:00 [accepted]
PHST- 2012/05/10 06:00 [entrez]
PHST- 2012/05/10 06:00 [pubmed]
PHST- 2013/09/14 06:00 [medline]
AID - 10.1007/s10571-012-9850-2 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2012 Nov;32(8):1255-63. doi: 10.1007/s10571-012-9850-2. Epub 
      2012 May 9.