PMID- 22571497
OWN - NLM
STAT- MEDLINE
DCOM- 20121010
LR  - 20171116
IS  - 1399-6576 (Electronic)
IS  - 0001-5172 (Linking)
VI  - 56
IP  - 6
DP  - 2012 Jul
TI  - Impact of shed blood products on stimulated cytokine release in an in vitro model 
      of transfusion.
PG  - 724-9
LID - 10.1111/j.1399-6576.2012.02704.x [doi]
AB  - BACKGROUND: Blood transfusion is reported to suppress the recipient's immune 
      system. To avoid allogenic transfusion, post-operative shed blood retransfusion 
      is a commonly used method. The aim of this study was to investigate the 
      dose-related impact of post-operatively collected shed blood products on the 
      stimulated cytokine release in an in vitro model of transfusion. METHODS: Venous 
      blood samples obtained from 20 patients undergoing hip arthroplasty were mixed 
      with post-operatively collected unprocessed, processed, and irradiated shed blood 
      as well as normal saline as a control. Shed blood was processed by centrifugation 
      and separating the cellular fraction from the soluble fraction and washing the 
      cellular fraction with phosphate buffered saline to eliminate any cell fragments 
      and other substances. Mixing ratios were 1:3, 1:1, and 3:1. Endotoxin-stimulated 
      release of Tumor Necrosis Factor-alpha (TNF-alpha) was measured after 24 h of culture 
      by enzyme-linked immunosorbent assay. RESULTS: Unprocessed, irradiated shed blood 
      and the soluble fraction caused a significant suppression of stimulated TNF-alpha 
      release compared to control. The addition of the cellular shed blood fraction had 
      no significant influence on the TNF-alpha release compared to control. CONCLUSION: 
      Shed blood and its components caused a dose-independent immunomodulation as 
      indicated by a suppressed stimulated TNF-alpha release. Leukocytes seem to play a 
      minor role, as we observed a sustained suppression after transfusion of 
      gamma-irradiated shed blood. Only the elimination of soluble factors by 
      centrifugation and followed by an additional washing step prevented the observed 
      suppression of TNF-alpha. Thus, we assume that washing of shed blood can prevent 
      potential detrimental effects.
CI  - (c) 2012 The Authors. Acta Anaesthesiologica Scandinavica (c) 2012 The Acta 
      Anaesthesiologica Scandinavica Foundation.
FAU - Schneider, S O
AU  - Schneider SO
AD  - Department for Anaesthesiology, Critical Care Medicine and Pain Therapy, Saarland 
      University Hospital, Homburg, Germany. sven.schneider@uks.eu
FAU - Biedler, A E
AU  - Biedler AE
FAU - Behmenburg, F
AU  - Behmenburg F
FAU - Volk, T
AU  - Volk T
FAU - Rensing, H
AU  - Rensing H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120509
PL  - England
TA  - Acta Anaesthesiol Scand
JT  - Acta anaesthesiologica Scandinavica
JID - 0370270
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Arthroplasty, Replacement, Hip
MH  - Blood/radiation effects
MH  - Centrifugation
MH  - Cytokines/analysis/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gamma Rays
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/metabolism
MH  - *Operative Blood Salvage
MH  - Stimulation, Chemical
MH  - Subcellular Fractions/physiology
MH  - *Transfusion Reaction
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2012/05/11 06:00
MHDA- 2012/10/12 06:00
CRDT- 2012/05/11 06:00
PHST- 2012/02/21 00:00 [accepted]
PHST- 2012/05/11 06:00 [entrez]
PHST- 2012/05/11 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
AID - 10.1111/j.1399-6576.2012.02704.x [doi]
PST - ppublish
SO  - Acta Anaesthesiol Scand. 2012 Jul;56(6):724-9. doi: 
      10.1111/j.1399-6576.2012.02704.x. Epub 2012 May 9.