PMID- 22571579 OWN - NLM STAT- MEDLINE DCOM- 20121030 LR - 20120510 IS - 1440-0960 (Electronic) IS - 0004-8380 (Linking) VI - 53 IP - 2 DP - 2012 May TI - Effect of HSPC016 gene expression on the aggregative growth of dermal papillae cells. PG - e26-9 LID - 10.1111/j.1440-0960.2010.00700.x [doi] AB - In previous studies, HSPC016 was identified as a differentially expressed gene in dermal papilla cells (DPC) with aggregative behaviour. To clarify its role in the regulation of DPC, the recombinant HSPC016 protein was expressed using the Pichia pastoris expression system, and three small interfering ribonucleic acid duplexes (siRNA) were designed and transfected into DPC. Results showed that DPC with the HSPC016 gene inhibited, exhibit non-aggregative behaviour and grow much slower than normal DPC, and that the recombinant HSPC016 protein could promote the proliferation of high-passage DPC and induce its aggregative behaviour. CI - (c) 2010 The Authors. Australasian Journal of Dermatology (c) 2010 The Australasian College of Dermatologists. FAU - Song, Zhiqiang AU - Song Z AD - Department of Dermatology, Southwest Hospital, Chongqing, China. FAU - Zhou, Chunli AU - Zhou C FAU - Wang, Jiwen AU - Wang J FAU - Yang, Weibin AU - Yang W FAU - Hao, Fei AU - Hao F LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20101013 PL - Australia TA - Australas J Dermatol JT - The Australasian journal of dermatology JID - 0135232 RN - 0 (Proteins) RN - 0 (TMA7 protein, human) SB - IM MH - Cell Aggregation/genetics MH - Cell Proliferation MH - Cells, Cultured MH - *Gene Expression MH - Hair Follicle/*cytology/metabolism MH - Humans MH - Proteins/*genetics EDAT- 2012/05/11 06:00 MHDA- 2012/10/31 06:00 CRDT- 2012/05/11 06:00 PHST- 2012/05/11 06:00 [entrez] PHST- 2012/05/11 06:00 [pubmed] PHST- 2012/10/31 06:00 [medline] AID - 10.1111/j.1440-0960.2010.00700.x [doi] PST - ppublish SO - Australas J Dermatol. 2012 May;53(2):e26-9. doi: 10.1111/j.1440-0960.2010.00700.x. Epub 2010 Oct 13.