PMID- 22571670
OWN - NLM
STAT- MEDLINE
DCOM- 20120827
LR  - 20190116
IS  - 1521-0553 (Electronic)
IS  - 0894-1939 (Linking)
VI  - 25
IP  - 4
DP  - 2012 Aug
TI  - Influence of hematopoietic stem cell-derived hepatocytes on liver regeneration 
      after sex-mismatched liver transplantation in humans.
PG  - 220-6
LID - 10.3109/08941939.2011.627088 [doi]
AB  - BACKGROUND: Presence of hematopoietic stem-cell-derived hepatocytes after 
      clinical liver transplantation was demonstrated repeatedly. The relevance of this 
      controversial mechanism of regeneration was discussed. Regarding frequency, the 
      demonstrated results were divergent. In the present study, we propose to 
      investigate the influence of growth and regeneration on the frequency of 
      hematopoietic stem-cell-derived hepatocytes in transplanted organs. MATERIAL AND 
      METHOD: Paraffin-embedded liver specimens, obtained as clinically indicated, from 
      female grafts transplanted into male recipients were investigated. The presence 
      of Y-chromosome in hepatocytes, detected by fluorescence in situ hybridization 
      (FISH), was the indicator for recipient origin. Slides were evaluated by 
      assessing the relative number of Y-chromosome containing hepatocytes within 50 
      images representing an average of 775 hepatocytes. RESULTS: In only 9 out of 81 
      specimens, single Y-chromosome positive hepatocytes were detected, resulting in a 
      maximal frequency of 0.64%. Six positive specimens were obtained from full-size 
      liver grafts and one from a partial liver graft. In the pediatric group, two 
      positive samples were found. By staining additional sections from the nine 
      positive specimens, no additional positive hepatocytes were detected suggesting 
      an even lower frequency within the whole sample. We did not find any accumulation 
      of Y-chromosome positive cells in any of the individually analyzed patient 
      groups. CONCLUSION: Transdifferentiation of hematopoietic stem cells is an 
      extremely rare event in liver growth and regeneration after transplantation. Due 
      to the low number of positive events, the biological relevance seems 
      questionable.
FAU - Schwerfeld-Bohr, Julia
AU  - Schwerfeld-Bohr J
AD  - Department of General, Visceral and Transplantation Surgery, University Hospital 
      Essen, Essen, Germany.
FAU - Chi, Haidong
AU  - Chi H
FAU - Worm, Karl
AU  - Worm K
FAU - Dahmen, Uta
AU  - Dahmen U
LA  - eng
PT  - Journal Article
DEP - 20120509
PL  - United States
TA  - J Invest Surg
JT  - Journal of investigative surgery : the official journal of the Academy of 
      Surgical Research
JID - 8809255
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Transdifferentiation
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Y
MH  - Female
MH  - Hematopoietic Stem Cells/*cytology
MH  - Hepatocytes/*physiology
MH  - Humans
MH  - *Liver Regeneration
MH  - *Liver Transplantation
MH  - Male
MH  - Middle Aged
EDAT- 2012/05/11 06:00
MHDA- 2012/08/28 06:00
CRDT- 2012/05/11 06:00
PHST- 2012/05/11 06:00 [entrez]
PHST- 2012/05/11 06:00 [pubmed]
PHST- 2012/08/28 06:00 [medline]
AID - 10.3109/08941939.2011.627088 [doi]
PST - ppublish
SO  - J Invest Surg. 2012 Aug;25(4):220-6. doi: 10.3109/08941939.2011.627088. Epub 2012 
      May 9.