PMID- 22572846
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20200930
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Linking)
VI  - 303
IP  - 2
DP  - 2012 Jul 15
TI  - Lipopolysaccharide-sensitive H+ current in dendritic cells.
PG  - C204-12
LID - 10.1152/ajpcell.00059.2012 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells equipped to 
      transport antigens from the periphery to lymphoid tissues and to present them to 
      T cells. Ligation of Toll-like receptor 4 (TLR4), expressed on the DC surface, by 
      lipopolysaccharides (LPS), elements of the Gram-negative bacteria outer wall, 
      induces DC maturation. Initial steps of maturation include stimulation of antigen 
      endocytosis and enhanced reactive oxygen species (ROS) production with eventual 
      downregulation of endocytic capacity in fully matured DCs. ROS production depends 
      on NADPH oxidase (NOX2), the activity of which requires continuous pH and charge 
      compensation. The present study demonstrates, for the first time, the functional 
      expression of voltage-gated proton (Hv1) channels in mouse bone marrow-derived 
      DCs. In whole cell patch-clamp experiments, we recorded Zn(2+) (50 muM)-sensitive 
      outwardly rectifying currents activated upon depolarization, which were highly 
      selective for H(+), with the reversal potential shift of 38 mV per pH unit. The 
      threshold voltage of activation (V(threshold)) was dependent on the pH gradient 
      and was close to the empirically predicted V(threshold) for the Hv1 currents. LPS 
      (1 mug/ml) had bimodal effects on Hv1 channels: acute LPS treatment increased Hv1 
      channel activity, whereas 24 h of LPS incubation significantly inhibited Hv1 
      currents and decreased ROS production. Activation of H(+) currents by acute 
      application of LPS was abolished by PKC inhibitor GFX (10 nM). According to 
      electron current measurements, acute LPS application was associated with 
      increased NOX2 activity.
FAU - Szteyn, Kalina
AU  - Szteyn K
AD  - Department of Physiology, University of Tubingen, Germany.
FAU - Yang, Wenting
AU  - Yang W
FAU - Schmid, Evi
AU  - Schmid E
FAU - Lang, Florian
AU  - Lang F
FAU - Shumilina, Ekaterina
AU  - Shumilina E
LA  - eng
PT  - Journal Article
DEP - 20120509
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Hv1 proton channel, mouse)
RN  - 0 (Ion Channels)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Ion Channels/*biosynthesis
MH  - Lipopolysaccharides/*pharmacology
MH  - Mice
MH  - Mice, 129 Strain
MH  - Mice, Inbred C57BL
MH  - Reactive Oxygen Species/metabolism
EDAT- 2012/05/11 06:00
MHDA- 2012/10/02 06:00
CRDT- 2012/05/11 06:00
PHST- 2012/05/11 06:00 [entrez]
PHST- 2012/05/11 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
AID - ajpcell.00059.2012 [pii]
AID - 10.1152/ajpcell.00059.2012 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2012 Jul 15;303(2):C204-12. doi: 
      10.1152/ajpcell.00059.2012. Epub 2012 May 9.