PMID- 22573306
OWN - NLM
STAT- MEDLINE
DCOM- 20130114
LR  - 20211021
IS  - 1098-1136 (Electronic)
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 60
IP  - 9
DP  - 2012 Sep
TI  - Differential distribution of diacylglycerol lipase-alpha and 
      N-acylphosphatidylethanolamine-specific phospholipase d immunoreactivity in the 
      superficial spinal dorsal horn of rats.
PG  - 1316-29
LID - 10.1002/glia.22351 [doi]
AB  - It is generally accepted that the endocannabinoid system plays important roles in 
      spinal pain processing. Although it is documented that cannabinoid-1 receptors 
      are strongly expressed in the superficial spinal dorsal horn, the cellular 
      distribution of enzymes that can synthesize endocannabinoid ligands is less well 
      studied. Thus, using immunocytochemical methods at the light and electron 
      microscopic levels, we investigated the distribution of diacylglycerol 
      lipase-alpha (DGL-alpha) and N-acylphosphatidylethanolamine-specific phospholipase D 
      (NAPE-PLD), enzymes synthesizing the endocannabinoid ligands, 
      2-arachidonoylglycerol (2-AG) and anandamide, respectively. Positive labeling was 
      revealed only occasionally in axon terminals, but dendrites displayed strong 
      immunoreactivity for both enzymes. However, the dendritic localization of DGL-alpha 
      and NAPE-PLD showed a remarkably different distribution. DGL-alpha immunolabeling in 
      dentrites was always revealed at membrane compartments in close vicinity to 
      synapses. In contrast to this, dendritic NAPE-PLD labeling was never observed in 
      association with synaptic contacts. In addition to dendrites, a substantial 
      proportion of astrocytic (immunoreactive for GFAP) and microglial (immunoreactive 
      for CD11b) profiles were also immunolabeled for both DGL-alpha and NAPE-PLD. Glial 
      processes immunostained for DGL-alpha were frequently found near to synapses in which 
      the postsynaptic dendrite was immunoreactive for DGL-alpha, whereas NAPE-PLD 
      immunoreactivity on glial profiles at the vicinity of synapses was only 
      occasionally observed. Our results suggest that both neurons and glial cells can 
      synthesize and release 2-AG and anandamide in the superficial spinal dorsal horn. 
      The 2-AG can primarily be released by postsynaptic dendrites and glial processes 
      adjacent to synapses, whereas anandamide can predominantly be released from 
      nonsynaptic dendritic and glial compartments.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Hegyi, Zoltan
AU  - Hegyi Z
AD  - Department of Anatomy, Histology and Embryology, Faculty of Medicine, Medical and 
      Health Science Center, University of Debrecen, Debrecen, Hungary.
FAU - Hollo, Krisztina
AU  - Hollo K
FAU - Kis, Greta
AU  - Kis G
FAU - Mackie, Ken
AU  - Mackie K
FAU - Antal, Miklos
AU  - Antal M
LA  - eng
GR  - K05 DA021696/DA/NIDA NIH HHS/United States
GR  - R01 DA011322/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120509
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
RN  - EC 3.1.4.4 (Napepld protein, rat)
RN  - EC 3.1.4.4 (Phospholipase D)
SB  - IM
MH  - Animals
MH  - Astrocytes/*enzymology/ultrastructure
MH  - Dendrites/enzymology/ultrastructure
MH  - Lipoprotein Lipase/*metabolism
MH  - Microglia/*enzymology/ultrastructure
MH  - Neurons/enzymology/ultrastructure
MH  - Phospholipase D/*metabolism
MH  - Rats
MH  - Rats, Inbred WKY
MH  - Spinal Cord/*enzymology/ultrastructure
MH  - Synapses/enzymology/ultrastructure
PMC - PMC3703832
MID - NIHMS479548
EDAT- 2012/05/11 06:00
MHDA- 2013/01/15 06:00
PMCR- 2013/09/01
CRDT- 2012/05/11 06:00
PHST- 2012/01/04 00:00 [received]
PHST- 2012/04/20 00:00 [accepted]
PHST- 2012/05/11 06:00 [entrez]
PHST- 2012/05/11 06:00 [pubmed]
PHST- 2013/01/15 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - 10.1002/glia.22351 [doi]
PST - ppublish
SO  - Glia. 2012 Sep;60(9):1316-29. doi: 10.1002/glia.22351. Epub 2012 May 9.