PMID- 22575045
OWN - NLM
STAT- MEDLINE
DCOM- 20121108
LR  - 20131121
IS  - 1477-2213 (Electronic)
IS  - 1028-6020 (Linking)
VI  - 14
IP  - 8
DP  - 2012
TI  - Anti-PRRSV effect and mechanism of sodium tanshinone IIA sulfonate in vitro.
PG  - 721-8
LID - 10.1080/10286020.2012.685727 [doi]
AB  - This experiment was conducted to study the antiviral activities of sodium 
      tanshinone IIA sulfonate (STS) against porcine reproductive and respiratory 
      syndrome virus (PRRSV) and its mechanism. Anti-PRRSV activities of STS were 
      observed on Marc-145 cells by using visualization of cytopathologic effect assay 
      and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, and 
      its antiviral mechanism was determined by time-of-addition assay, adsorption 
      inhibition assay, and virucidal assay. The results showed that STS could reduce 
      the damage of PRRSV to Marc-145 cells, with the inhibition ratio exceeding to 
      100%, at the maximum non-cytotoxic concentration. The time-of-addition and 
      virucidal assays indicated that the anti-PRRSV activities of STS could be due to 
      inhibiting the virus replication or/and inactivating the virus directly. The 
      inhibition of the virus attachment was not discovered in adsorption inhibition 
      assay. The results proved that STS had strong anti-PRRSV activity and encouraged 
      for further exploration of STS.
FAU - Sun, Na
AU  - Sun N
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu 030801, China.
FAU - Zhao, Xin
AU  - Zhao X
FAU - Bai, Xi-Yun
AU  - Bai XY
FAU - Niu, Li
AU  - Niu L
FAU - Song, Mei-Qin
AU  - Song MQ
FAU - Sun, Yao-Gui
AU  - Sun YG
FAU - Jiang, Jun-Bing
AU  - Jiang JB
FAU - Li, Hong-Quan
AU  - Li HQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120510
PL  - England
TA  - J Asian Nat Prod Res
JT  - Journal of Asian natural products research
JID - 100888334
RN  - 0 (Antiviral Agents)
RN  - 0 (Phenanthrenes)
RN  - 69659-80-9 (tanshinone II A sodium sulfonate)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/chemistry/*isolation & purification/*pharmacology
MH  - Humans
MH  - Molecular Structure
MH  - Phenanthrenes/chemistry/*pharmacology
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Sodium/pharmacology
MH  - Swine
EDAT- 2012/05/12 06:00
MHDA- 2012/11/09 06:00
CRDT- 2012/05/12 06:00
PHST- 2012/05/12 06:00 [entrez]
PHST- 2012/05/12 06:00 [pubmed]
PHST- 2012/11/09 06:00 [medline]
AID - 10.1080/10286020.2012.685727 [doi]
PST - ppublish
SO  - J Asian Nat Prod Res. 2012;14(8):721-8. doi: 10.1080/10286020.2012.685727. Epub 
      2012 May 10.