PMID- 22575897
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20200826
IS  - 1465-2080 (Electronic)
IS  - 1350-0872 (Linking)
VI  - 158
IP  - Pt 7
DP  - 2012 Jul
TI  - Identification of glucose-fermenting bacteria in a full-scale enhanced biological 
      phosphorus removal plant by stable isotope probing.
PG  - 1818-1825
LID - 10.1099/mic.0.058818-0 [doi]
AB  - Microbiology in wastewater treatment has mainly been focused on problem-causing 
      filamentous bacteria or bacteria directly involved in nitrogen and phosphorus 
      removal, and to a lesser degree on flanking groups, such as hydrolysing and 
      fermenting bacteria. However, these groups constitute important suppliers of 
      readily degradable substrates for the overall processes in the plant. This study 
      aimed to identify glucose-fermenting bacteria in a full-scale enhanced biological 
      phosphorus removal (EBPR) wastewater treatment plant (WWTP), and to determine 
      their abundance in similar WWTPs. Glucose-fermenting micro-organisms were 
      identified by an in situ approach using RNA-based stable isotope probing. 
      Activated sludge was incubated anaerobically with (13)C(6)-labelled glucose, and 
      (13)C-enriched rRNA was subsequently reverse-transcribed and used to construct a 
      16S rRNA gene clone library. Phylogenetic analysis of the library revealed the 
      presence of two major phylogenetic groups of gram-positive bacteria affiliating 
      with the genera Tetrasphaera, Propionicimonas (Actinobacteria), and Lactococcus 
      and Streptococcus (Firmicutes). Specific oligonucleotide probes were designed for 
      fluorescence in situ hybridization (FISH) to specifically target the 
      glucose-fermenting bacteria identified in this study. The combination of FISH 
      with microautoradiography confirmed that Tetrasphaera, Propionicimonas and 
      Streptococcus were the dominant glucose fermenters. The probe-defined fermenters 
      were quantified in 10 full-scale EBPR plants and averaged 39 % of the total 
      biovolume. Tetrasphaera and Propionicimonas were the most abundant glucose 
      fermenters (average 33 and 4 %, respectively), while Streptococcus and 
      Lactococcus were present only in some WWTPs (average 1 and 0.4 %, respectively). 
      Thus the population of actively metabolizing glucose fermenters seems to occupy a 
      relatively large component of the total biovolume.
FAU - Nielsen, Jeppe Lund
AU  - Nielsen JL
AD  - Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg 
      University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
FAU - Nguyen, Hien
AU  - Nguyen H
AD  - Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg 
      University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
FAU - Meyer, Rikke Louise
AU  - Meyer RL
AD  - Interdisciplinary Nanoscience Centre (iNANO) and Department of Biological 
      Sciences, University of Aarhus, Ny Munkegade, DK-8000 Aarhus C, Denmark.
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
AD  - Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg 
      University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
LA  - eng
SI  - GENBANK/JQ425742
SI  - GENBANK/JQ425743
SI  - GENBANK/JQ425744
SI  - GENBANK/JQ425745
SI  - GENBANK/JQ425746
SI  - GENBANK/JQ425747
SI  - GENBANK/JQ425748
SI  - GENBANK/JQ425749
SI  - GENBANK/JQ425750
SI  - GENBANK/JQ425751
SI  - GENBANK/JQ425752
SI  - GENBANK/JQ425753
SI  - GENBANK/JQ425754
SI  - GENBANK/JQ425755
SI  - GENBANK/JQ425756
SI  - GENBANK/JQ425757
SI  - GENBANK/JQ425758
SI  - GENBANK/JQ425759
SI  - GENBANK/JQ425760
SI  - GENBANK/JQ425761
SI  - GENBANK/JQ425762
SI  - GENBANK/JQ425763
SI  - GENBANK/JQ425764
SI  - GENBANK/JQ425765
SI  - GENBANK/JQ425766
SI  - GENBANK/JQ425767
SI  - GENBANK/JQ425768
SI  - GENBANK/JQ425769
SI  - GENBANK/JQ425770
SI  - GENBANK/JQ425771
SI  - GENBANK/JQ425772
SI  - GENBANK/JQ425773
SI  - GENBANK/JQ425774
SI  - GENBANK/JQ425775
SI  - GENBANK/JQ425776
SI  - GENBANK/JQ425777
SI  - GENBANK/JQ425778
SI  - GENBANK/JQ425779
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120510
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Carbon Isotopes)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 27YLU75U4W (Phosphorus)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Bacteria/*classification/*metabolism
MH  - *Biodiversity
MH  - Carbon Isotopes/metabolism
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fermentation
MH  - Glucose/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Isotope Labeling
MH  - Metagenome
MH  - Molecular Sequence Data
MH  - Phosphorus/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Water Microbiology
MH  - Water Purification/methods
EDAT- 2012/05/12 06:00
MHDA- 2012/11/02 06:00
CRDT- 2012/05/12 06:00
PHST- 2012/05/12 06:00 [entrez]
PHST- 2012/05/12 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
AID - 10.1099/mic.0.058818-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2012 Jul;158(Pt 7):1818-1825. doi: 
      10.1099/mic.0.058818-0. Epub 2012 May 10.