PMID- 22576851
OWN - NLM
STAT- MEDLINE
DCOM- 20121019
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 100
IP  - 5
DP  - 2012 Jul
TI  - Experimental repair of segmental bone defects in rabbits by angiopoietin-1 gene 
      transfected MSCs seeded on porous beta-TCP scaffolds.
PG  - 1229-36
LID - 10.1002/jbm.b.32687 [doi]
AB  - Segmental bone defect repair remains a clinical and experimental challenge in 
      tissue engineering with increasing focus on angiogenesis in the bone substitutes. 
      The objective of this study was to investigate the osteogenic effects of 
      angiopoietin-1 (Ang-1) gene transfected bone marrow-derived mesenchymal stem 
      cells (MSCs) seeded on porous beta-TCP scaffolds. This bone substitute (experimental 
      group) and MSCs/beta-TCP compounds (control group) were implanted into 15 mm 
      segmental bone defects of the radii of 30 New Zealand white rabbits, with 
      platelet-rich plasma injected at the same time. Bone regeneration and 
      angiogenesis were assessed by Scanning electron microscope (SEM), X-ray, 
      histology, immunohistology, and biomechanical outcome measurements made on the 
      2nd, 4th, 8th, and 12th week after the operation. In vitro, the amount of 
      proliferation and differentiation of Ang-1 gene transfected MSCs was found to be 
      gross increased than that of the control groups. In vivo, a significantly 
      increased amount of new bone formation accompanied by active capillary 
      vasculature regeneration was observed in the pores of the scaffolds which had 
      been seeded with Ang-1 gene transfected MSCs, as compared with the control 
      groups. The biomechanical test confirmed the failure load of new born bone was 
      close to normal bone. These results suggest that transfer of gene encoding Ang-1 
      to MSCs increases their osteogenic properties by enhancing capillary 
      regeneration, thus providing a rich blood supply for new bone formation in 
      segmental bone defects.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Cao, Le
AU  - Cao L
AD  - Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiaotong University, Shanghai 200233, People's Republic of China.
FAU - Liu, Xudong
AU  - Liu X
FAU - Liu, Shen
AU  - Liu S
FAU - Jiang, Yao
AU  - Jiang Y
FAU - Zhang, Xianlong
AU  - Zhang X
FAU - Zhang, Changqing
AU  - Zhang C
FAU - Zeng, Bingfang
AU  - Zeng B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120511
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Angiopoietin-1)
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
SB  - IM
MH  - Angiopoietin-1/*biosynthesis/genetics
MH  - Animals
MH  - *Bone Regeneration
MH  - Calcium Phosphates/*chemistry
MH  - Cells, Cultured
MH  - Fractures, Bone/metabolism/pathology/*therapy
MH  - Mesenchymal Stem Cells/*metabolism
MH  - *Neovascularization, Physiologic
MH  - Porosity
MH  - Rabbits
MH  - *Tissue Scaffolds
MH  - Transfection
EDAT- 2012/05/12 06:00
MHDA- 2012/10/20 06:00
CRDT- 2012/05/12 06:00
PHST- 2011/01/19 00:00 [accepted]
PHST- 2011/05/17 00:00 [received]
PHST- 2011/12/17 00:00 [revised]
PHST- 2012/05/12 06:00 [entrez]
PHST- 2012/05/12 06:00 [pubmed]
PHST- 2012/10/20 06:00 [medline]
AID - 10.1002/jbm.b.32687 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2012 Jul;100(5):1229-36. doi: 
      10.1002/jbm.b.32687. Epub 2012 May 11.