PMID- 22579699
OWN - NLM
STAT- MEDLINE
DCOM- 20121016
LR  - 20120618
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 59
IP  - 2
DP  - 2012 Aug
TI  - Predominance of IL-10 and TGF-beta production from the mouse macrophage cell line, 
      RAW264.7, in response to crude antigens from Clonorchis sinensis.
PG  - 237-44
LID - 10.1016/j.cyto.2012.04.021 [doi]
AB  - Parasitic helminths are well-known to have the ability to modulate host immune 
      responses. In this study, we investigated the fundamental immunoregulatory 
      mechanism of the liver fluke Clonorchis sinensis (C. sinensis) using a murine 
      macrophage RAW 264.7 (RAW) cell line and mouse bone marrow derived macrophages 
      (BMDMs). We found that C. sinensis crude antigen (CA) is able to differentiate 
      macrophage RAW cells into dendritic-like cells that can be detected by 
      morphological observations. In addition, CA induces prominent secretion of 
      anti-inflammatory cytokines such as IL-10 and TGF-beta; however, we did not observe 
      changes in cell surface markers that are involved in antigen recognition, antigen 
      presentation, and T cell activation. Additionally, CA treatment induced ERK and 
      JNK phosphorylation, and unexpectedly, elevated levels of IL-10 and TGF-beta were 
      inhibited by the addition of an ERK-specific inhibitor. Taken together, these 
      data demonstrate that CA from C. sinensis may modulate host immune responses by 
      upregulating anti-inflammatory cytokines via the regulation of ERK.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Wi, Hae Joo
AU  - Wi HJ
AD  - Department of Parasitology and Tropical Medicine, Seoul National University 
      College of Medicine, Seoul, South Korea.
FAU - Jin, Yan
AU  - Jin Y
FAU - Choi, Min-Ho
AU  - Choi MH
FAU - Hong, Sung-Tae
AU  - Hong ST
FAU - Bae, Young Mee
AU  - Bae YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120512
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antigens, Helminth)
RN  - 0 (Antigens, Surface)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/*immunology
MH  - Antigens, Surface/metabolism
MH  - Bone Marrow Cells/cytology/drug effects
MH  - Cell Line
MH  - Cell Shape/drug effects
MH  - Clonorchis sinensis/*immunology
MH  - Dendritic Cells/cytology/drug effects/immunology
MH  - Enzyme Activation/drug effects
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Humans
MH  - Interleukin-10/*biosynthesis
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Macrophages/drug effects/enzymology/*immunology/*parasitology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Transforming Growth Factor beta/*biosynthesis
MH  - Up-Regulation/drug effects
EDAT- 2012/05/15 06:00
MHDA- 2012/10/17 06:00
CRDT- 2012/05/15 06:00
PHST- 2010/11/25 00:00 [received]
PHST- 2012/02/24 00:00 [revised]
PHST- 2012/04/17 00:00 [accepted]
PHST- 2012/05/15 06:00 [entrez]
PHST- 2012/05/15 06:00 [pubmed]
PHST- 2012/10/17 06:00 [medline]
AID - S1043-4666(12)00151-2 [pii]
AID - 10.1016/j.cyto.2012.04.021 [doi]
PST - ppublish
SO  - Cytokine. 2012 Aug;59(2):237-44. doi: 10.1016/j.cyto.2012.04.021. Epub 2012 May 
      12.