PMID- 22581583
OWN - NLM
STAT- MEDLINE
DCOM- 20130605
LR  - 20240404
IS  - 1520-6777 (Electronic)
IS  - 0733-2467 (Print)
IS  - 0733-2467 (Linking)
VI  - 32
IP  - 1
DP  - 2013 Jan
TI  - Neurotrophin therapy improves recovery of the neuromuscular continence mechanism 
      following simulated birth injury in rats.
PG  - 82-7
LID - 10.1002/nau.22264 [doi]
AB  - AIMS: Stress urinary incontinence (SUI) affects women both acutely and 
      chronically after vaginal delivery. Current SUI treatments assume the 
      neuromuscular continence mechanism, comprised of the pudendal nerve (PN) and 
      external urethral sphincter (EUS), is either intact or irreparable. This study 
      investigated the ability of neurotrophin therapy to facilitate recovery of the 
      neuromuscular continence mechanism. METHODS: Virgin, Sprague Dawley rats received 
      simulated childbirth injury or sham injury and treatment with continuous infusion 
      of brain-derived neurotrophic factor (BDNF) or saline placebo to the site of PN 
      injury. Continence was assessed by leak point pressure (LPP) and EUS 
      electromyography (EMG) 14 and 21 days after injury. Structural recovery was 
      assessed histologically. Molecular assessment of the muscular and 
      neuroregenerative response was determined via measurement of EUS BDNF and PN 
      beta(II) -tubulin expression respectively, 4, 8, and 12 days after injury. RESULTS: 
      Following injury, LPP was significantly reduced with saline compared to either 
      BDNF treatment or sham injury. Similarly, compared to sham injury, resting EUS 
      EMG amplitude and firing rate, as well as amplitude during LPP were significantly 
      reduced with saline but not BDNF treatment. Histology confirmed improved EUS 
      recovery with BDNF treatment. EUS BDNF and PN beta(II)-tubulin expression 
      demonstrated that BDNF treatment improved the neurogenerative response and may 
      facilitate sphincteric recovery. CONCLUSIONS: Continuous targeted neurotrophin 
      therapy accelerates continence recovery after simulated childbirth injury likely 
      through stimulating neuroregeneration and facilitating EUS recovery and 
      re-innervation. Neurotrophins or other therapies targeting neuromuscular 
      regeneration may be useful for treating SUI related to failure of the 
      neuromuscular continence mechanism.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Gill, Bradley C
AU  - Gill BC
AD  - Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, 
      Cleveland, Ohio, USA.
FAU - Balog, Brian M
AU  - Balog BM
FAU - Dissaranan, Charuspong
AU  - Dissaranan C
FAU - Jiang, Hai-Hong
AU  - Jiang HH
FAU - Steward, James B
AU  - Steward JB
FAU - Lin, Dan Li
AU  - Lin DL
FAU - Damaser, Margot S
AU  - Damaser MS
LA  - eng
GR  - R01 HD038679/HD/NICHD NIH HHS/United States
GR  - R01 HD038679-11/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120511
PL  - United States
TA  - Neurourol Urodyn
JT  - Neurourology and urodynamics
JID - 8303326
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology/*therapeutic use
MH  - Delivery, Obstetric/*adverse effects
MH  - Disease Models, Animal
MH  - Electromyography
MH  - Female
MH  - Nerve Crush
MH  - Peripheral Nerve Injuries/*drug therapy/etiology/physiopathology
MH  - Pudendal Nerve/injuries
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/drug effects/physiology
MH  - Treatment Outcome
MH  - Urinary Incontinence, Stress/*drug therapy/etiology/physiopathology
MH  - Vagina/*injuries
PMC - PMC3419785
MID - NIHMS371372
EDAT- 2012/05/15 06:00
MHDA- 2013/06/06 06:00
PMCR- 2014/01/01
CRDT- 2012/05/15 06:00
PHST- 2011/12/16 00:00 [received]
PHST- 2012/04/03 00:00 [accepted]
PHST- 2012/05/15 06:00 [entrez]
PHST- 2012/05/15 06:00 [pubmed]
PHST- 2013/06/06 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.1002/nau.22264 [doi]
PST - ppublish
SO  - Neurourol Urodyn. 2013 Jan;32(1):82-7. doi: 10.1002/nau.22264. Epub 2012 May 11.