PMID- 22582060 OWN - NLM STAT- MEDLINE DCOM- 20121126 LR - 20240412 IS - 1098-5336 (Electronic) IS - 0099-2240 (Print) IS - 0099-2240 (Linking) VI - 78 IP - 14 DP - 2012 Jul TI - Associations of yeasts with spotted-wing Drosophila (Drosophila suzukii; Diptera: Drosophilidae) in cherries and raspberries. PG - 4869-73 LID - 10.1128/AEM.00841-12 [doi] AB - A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal restriction fragment length polymorphism (TRFLP) technology and decimal dilution plating were used to identify and determine the relative abundance of yeast species present in fruit juice samples that were either infested with D. suzukii or not infested. Yeasts were less abundant in uninfested than infested samples. A total of 126 independent yeast isolates were cultivated from frass, midguts, and fruit hosts of D. suzukii, representing 28 species of yeasts, with Hanseniaspora uvarum predominating. This suggests an association between D. suzukii and H. uvarum that could be utilized for pest management of the highly pestiferous D. suzukii. FAU - Hamby, Kelly A AU - Hamby KA AD - Department of Entomology and Nematology, University of California, Davis, Davis, CA, USA. kahamby@ucdavis.edu FAU - Hernandez, Alejandro AU - Hernandez A FAU - Boundy-Mills, Kyria AU - Boundy-Mills K FAU - Zalom, Frank G AU - Zalom FG LA - eng PT - Journal Article DEP - 20120511 PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 SB - IM MH - Animals MH - Crops, Agricultural/growth & development/*parasitology MH - Drosophila/classification/growth & development/*microbiology MH - Fruit/growth & development/*parasitology MH - Plant Diseases/parasitology MH - Polymorphism, Restriction Fragment Length MH - Saccharomycetales/genetics/growth & development/isolation & purification MH - Symbiosis MH - Yeasts/classification/genetics/*growth & development/isolation & purification PMC - PMC3416361 EDAT- 2012/05/15 06:00 MHDA- 2012/12/10 06:00 PMCR- 2013/01/01 CRDT- 2012/05/15 06:00 PHST- 2012/05/15 06:00 [entrez] PHST- 2012/05/15 06:00 [pubmed] PHST- 2012/12/10 06:00 [medline] PHST- 2013/01/01 00:00 [pmc-release] AID - AEM.00841-12 [pii] AID - 00841-12 [pii] AID - 10.1128/AEM.00841-12 [doi] PST - ppublish SO - Appl Environ Microbiol. 2012 Jul;78(14):4869-73. doi: 10.1128/AEM.00841-12. Epub 2012 May 11.