PMID- 22582139
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1948-7193 (Electronic)
IS  - 1948-7193 (Print)
IS  - 1948-7193 (Linking)
VI  - 3
IP  - 1
DP  - 2012 Jan 18
TI  - Small Molecule Induction of Human Umbilical Stem Cells into MBP-positive 
      Oligodendrocytes in a Defined Three-Dimensional Environment.
PG  - 31-39
AB  - Stem cells from umbilical cord would be a favorable alternative to embryonic stem 
      cells for therapeutic applications. In this study, human multipotent progenitor 
      cells (MLPCs) from umbilical cord were differentiated into oligodendrocytes by 
      exposure to a range of microenvironmental chemical and physical cues. Chemical 
      cues were represented by a novel defined differentiation medium containing the 
      neurotransmitter norepinephrine (NE). In traditional 2 dimensional (2D) 
      conditions, the MLPCs differentiated into oligodendrocyte precursors, but did not 
      progress further. However, in a 3 dimensional (3D) environment, the MLPCs 
      differentiated into committed oligodendrocytes that expressed MBP. The apparent 
      method of interaction of NE in stimulating the differentiation process was 
      identified to occur through the adenergic pathway while all prior differentiation 
      methods have used other routes. This novel method of obtaining functional human 
      oligodendrocytes from MLPCs would eliminate many of the difficulties associated 
      with their differentiation from embryonic stem cells.
FAU - Davis, Hedvika
AU  - Davis H
AD  - Hybrid Systems Laboratory, NanoScience Technology Center, University of Central 
      Florida, Orlando, FL 32826, USA.
FAU - Guo, Xiufang
AU  - Guo X
FAU - Lambert, Stephen
AU  - Lambert S
FAU - Stancescu, Maria
AU  - Stancescu M
FAU - Hickman, James J
AU  - Hickman JJ
LA  - eng
GR  - R01 NS050452/NS/NINDS NIH HHS/United States
GR  - R01 NS050452-05/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20111129
PL  - United States
TA  - ACS Chem Neurosci
JT  - ACS chemical neuroscience
JID - 101525337
PMC - PMC3349170
MID - NIHMS337556
EDAT- 2012/05/15 06:00
MHDA- 2012/05/15 06:01
PMCR- 2013/01/18
CRDT- 2012/05/15 06:00
PHST- 2012/05/15 06:00 [entrez]
PHST- 2012/05/15 06:00 [pubmed]
PHST- 2012/05/15 06:01 [medline]
PHST- 2013/01/18 00:00 [pmc-release]
AID - 10.1021/cn200082q [doi]
PST - ppublish
SO  - ACS Chem Neurosci. 2012 Jan 18;3(1):31-39. doi: 10.1021/cn200082q. Epub 2011 Nov 
      29.