PMID- 22582269
OWN - NLM
STAT- MEDLINE
DCOM- 20120910
LR  - 20120514
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 36
IP  - 5
DP  - 2012 Jun
TI  - Comparison of five derivatizing agents for the determination of amphetamine-type 
      stimulants in human urine by extractive acylation and gas chromatography-mass 
      spectrometry.
PG  - 340-4
LID - 10.1093/jat/bks026 [doi]
AB  - Five acylation reagents have been compared for use as derivatizing agents for the 
      analysis of amphetamine-type stimulants (ATS) in urine by gas chromatography-mass 
      spectrometry (GC-MS). The evaluated reagents were heptafluorobutyric anhydride, 
      pentafluoropropionic anhydride, trifluoroacetic anhydride, acetic anhydride (AA) 
      and N-methyl-bis(trifluoroacetamide). The ATS included amphetamine, 
      methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 
      3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyethylamphetamine 
      (MDEA). A mixture of the ATS was added to urine (1 mL) followed by KOH solution 
      and saturated NaHCO(3) solution. The sample was then extracted with 
      dichloromethane and the derivatizing agent and 2 microL were injected into the GC-MS 
      instrument. The derivatizing agents were compared with reference to the 
      signal-to-noise (S/N) ratios, peak area values, relative standard deviations 
      (RSDs), linearities, limits of detection (LODs) and selectivities. The acetic 
      anhydride proved to be the best according to the S/N ratio and peak area results 
      for amphetamine, MA, MDMA and MDEA. The best RSD values of peak areas and of S/N 
      ratios at 3 microg/mL were also given by AA in cases of MDA, MDMA and MDEA. At 20 
      microg/mL, the lowest RSD values of peak areas for MDA and the lowest RSD values of 
      S/N ratios for MA, MDA, MDMA and MDEA were again given by AA. Additionally, the 
      highest correlation coefficients for MA, MDA, MDMA and MDEA and the lowest LOD 
      results for MA, MDMA and MDEA were produced by AA.
FAU - Dobos, Adrienn
AU  - Dobos A
AD  - National Institute of Forensic Toxicology, H-1146 Budapest, Varanno utca 2-4, 
      Hungary. dobosa@iszki.hu
FAU - Hidvegi, Elod
AU  - Hidvegi E
FAU - Somogyi, Gabor Pal
AU  - Somogyi GP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Stimulants)
SB  - IM
MH  - Acylation
MH  - Amphetamines/chemistry/*urine
MH  - Central Nervous System Stimulants/chemistry/*urine
MH  - Drug and Narcotic Control/methods
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Substance Abuse Detection/*methods
EDAT- 2012/05/15 06:00
MHDA- 2012/09/11 06:00
CRDT- 2012/05/15 06:00
PHST- 2012/05/15 06:00 [entrez]
PHST- 2012/05/15 06:00 [pubmed]
PHST- 2012/09/11 06:00 [medline]
AID - bks026 [pii]
AID - 10.1093/jat/bks026 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2012 Jun;36(5):340-4. doi: 10.1093/jat/bks026.