PMID- 22582814
OWN - NLM
STAT- MEDLINE
DCOM- 20130405
LR  - 20120926
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 41
IP  - 9
DP  - 2012 Oct
TI  - Transfection of hypoxia-inducible factor-1 decoy oligodeoxynucleotides suppresses 
      expression of vascular endothelial growth factor in oral squamous cell carcinoma 
      cells.
PG  - 675-81
LID - 10.1111/j.1600-0714.2012.01161.x [doi]
AB  - Vasculature development is thought to be an important aspect in the growth and 
      metastasis of solid tumors. Among the many angiogenic factors produced by tumor 
      cells, vascular endothelial growth factor (VEGF) is considered to play a key role 
      in angiogenic processes. VEGF synthesis is modulated by hypoxia-inducible 
      factor-1 (HIF-1) function within the hypoxic microenvironment of growing cancer 
      tissue. To inhibit HIF-1 activation, oligodeoxynucleotides (ODNs) were 
      synthesized and transferred with either the consensus sequence for HIF-1 binding 
      or a mutated form of this sequence. If we could transfer a large number of ODNs 
      into the cancer cell nucleus, activated HIF-1 might bind to the ODNs, resulting 
      in inhibition of hypoxia-induced VEGF synthesis. We transferred these ODNs into 
      cultured oral squamous cell carcinoma cells (SAS cells) using the 
      hemagglutinating virus of Japan (HVJ)-liposome method. Hypoxia-mediated 
      expression of VEGF by cancer cells was suppressed by transfection of HIF-1 decoy 
      ODNs, but not by mutated HIF-1 decoy ODNs. HIF-1 decoy ODN transfection also 
      inhibited VEGF protein synthesis. These results suggest that transfection with 
      HIF-1 decoy ODNs is effective for regulating tumor growth by reducing VEGF.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Imai, Mie
AU  - Imai M
AD  - Department of Oral and Maxillofacial Surgery, Graduate School of Dental Sciences, 
      Kyushu University, Fukuoka, Japan.
FAU - Ishibashi, Hiroaki
AU  - Ishibashi H
FAU - Nariai, Yoshiki
AU  - Nariai Y
FAU - Kanno, Takahiro
AU  - Kanno T
FAU - Sekine, Joji
AU  - Sekine J
FAU - Onimaru, Mitsuho
AU  - Onimaru M
FAU - Mori, Yoshihide
AU  - Mori Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120515
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Liposomes)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Carcinoma, Squamous Cell/genetics/*metabolism
MH  - Cell Culture Techniques
MH  - Cell Hypoxia/genetics
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism
MH  - Consensus Sequence/genetics
MH  - Cytoplasm/metabolism
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/*genetics
MH  - Liposomes
MH  - Mouth Neoplasms/genetics/*metabolism
MH  - Mutation/genetics
MH  - Neovascularization, Pathologic/genetics
MH  - Oligodeoxyribonucleotides/*genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Sendai virus/genetics
MH  - Transcriptional Activation/genetics
MH  - Transfection/*methods
MH  - Vascular Endothelial Growth Factor A/analysis/*metabolism
EDAT- 2012/05/16 06:00
MHDA- 2013/04/06 06:00
CRDT- 2012/05/16 06:00
PHST- 2012/05/16 06:00 [entrez]
PHST- 2012/05/16 06:00 [pubmed]
PHST- 2013/04/06 06:00 [medline]
AID - 10.1111/j.1600-0714.2012.01161.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2012 Oct;41(9):675-81. doi: 10.1111/j.1600-0714.2012.01161.x. 
      Epub 2012 May 15.