PMID- 22584270
OWN - NLM
STAT- MEDLINE
DCOM- 20121108
LR  - 20181201
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 184
IP  - 1-2
DP  - 2012 Sep
TI  - A high-throughput assay for HIV-1 integrase 3'-processing activity using 
      time-resolved fluorescence.
PG  - 34-40
LID - 10.1016/j.jviromet.2012.05.003 [doi]
AB  - HIV-1 integrase (HIV-1 IN), a well-validated antiviral drug target, catalyzes 
      multistep reactions to incorporate viral DNA into the genome of the host cell; 
      these include both a 3'-processing (3'P) reaction and a strand transfer reaction. 
      These enzymatic activities can be measured in vitro with short DNA 
      oligonucleotides that mimic a single viral LTR DNA end and purified IN. A highly 
      sensitive and reproducible time-resolved fluorescence (TRF)-based assay for HIV-1 
      IN 3'P activity is now reported. This assay was optimized with respect to time 
      and concentrations of metal ions, substrate and enzyme. The assay has now been 
      used successfully to measure HIV-1 IN 3'P activity and has been shown to detect 
      the anti-IN activity of several known 3'P inhibition compounds accurately. This 
      assay, which is amenable to high-throughput screening, will be useful for 
      identification of additional HIV-1 IN 3'P inhibitors.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Han, Ying-Shan
AU  - Han YS
AD  - McGill University AIDS Centre, Lady Davis for Medical Research, Jewish General 
      Hospital, Montreal, Quebec, Canada.
FAU - Quashie, Peter
AU  - Quashie P
FAU - Mesplede, Thibault
AU  - Mesplede T
FAU - Xu, Hongtao
AU  - Xu H
FAU - Mekhssian, Kevork
AU  - Mekhssian K
FAU - Fenwick, Craig
AU  - Fenwick C
FAU - Wainberg, Mark A
AU  - Wainberg MA
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120511
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (HIV Integrase Inhibitors)
RN  - EC 2.7.7.- (HIV Integrase)
RN  - YY6481J2FF (p31 integrase protein, Human immunodeficiency virus 1)
SB  - IM
MH  - *Fluorescence
MH  - HIV Integrase/*metabolism
MH  - HIV Integrase Inhibitors/*analysis
MH  - HIV-1/*enzymology
MH  - High-Throughput Screening Assays/*methods
MH  - Humans
MH  - Time Factors
EDAT- 2012/05/16 06:00
MHDA- 2012/11/09 06:00
CRDT- 2012/05/16 06:00
PHST- 2011/10/19 00:00 [received]
PHST- 2012/04/26 00:00 [revised]
PHST- 2012/05/03 00:00 [accepted]
PHST- 2012/05/16 06:00 [entrez]
PHST- 2012/05/16 06:00 [pubmed]
PHST- 2012/11/09 06:00 [medline]
AID - S0166-0934(12)00159-0 [pii]
AID - 10.1016/j.jviromet.2012.05.003 [doi]
PST - ppublish
SO  - J Virol Methods. 2012 Sep;184(1-2):34-40. doi: 10.1016/j.jviromet.2012.05.003. 
      Epub 2012 May 11.