PMID- 22590820
OWN - NLM
STAT- MEDLINE
DCOM- 20120919
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 34
IP  - 1
DP  - 2012 Feb
TI  - Micronucleus scoring in urine cytology specimen by DNA-specific stain.
PG  - 56-60
AB  - OBJECTIVE: To evaluate the role of micronuclei (MN) scoring in urinary cytology 
      of bladder transitional cell carcinoma (TCC). STUDY DESIGN: Eighty cases of 
      freshly voided urine samples were used for scoring of MN using acridine orange 
      (AO)-stained smears under fluorescence microscope and routine 
      Papanicolaou-stained smears under light microscopy. MN score for cytologic 
      lesions was compared. RESULTS: In AO-stained slides, the mean MN scores were 
      0.171 +/- 0.395 and 1.489 +/- 1.871 in smears with "no malignant cells seen" and 
      TCC cases, respectively. In Papanicolaou-stained slides the mean MN scores were 
      0.194 +/- 0.419 and 1.84 +/- 1.876 in smears with "no malignant cells seen," and 
      TCC cases, respectively. Independent sample t test showed significant difference 
      of MN scoring in Papanicolaou-stain and AO stain in no malignancy vs. TCC. 
      Pearson's correlation test showed strong positive correlation between mean MN 
      score by AO and Papanicolaou stains and Pearson's correlation coefficient. 
      CONCLUSION: MN score was significantly high in TCC cases, which may be due to 
      chromosomal damage in bladder carcinoma. In difficult cases, MN may help 
      distinguish benign from malignant cells in urine samples. Routine Papanicolaou 
      stain may also be used for MN score because DNA-specific AO-stained smear gives 
      almost similar results.
FAU - Sharma, Shelly
AU  - Sharma S
AD  - Department of Cytopathology and Gynecological Pathology, Postgraduate Institute 
      of Medical Education and Research, Chandigarh, India.
FAU - Dey, Pranab
AU  - Dey P
FAU - Saikia, Uma Nahar
AU  - Saikia UN
FAU - Nijhawan, Raje
AU  - Nijhawan R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*pathology
MH  - Humans
MH  - Light
MH  - Micronucleus Tests
MH  - Microscopy
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence
MH  - Staining and Labeling
MH  - Urinary Bladder Neoplasms/*pathology
MH  - Vaginal Smears
MH  - Young Adult
EDAT- 2012/05/18 06:00
MHDA- 2012/09/20 06:00
CRDT- 2012/05/18 06:00
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/09/20 06:00 [medline]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2012 Feb;34(1):56-60.