PMID- 22591661
OWN - NLM
STAT- MEDLINE
DCOM- 20121018
LR  - 20211021
IS  - 1523-5866 (Electronic)
IS  - 1522-8517 (Print)
IS  - 1522-8517 (Linking)
VI  - 14
IP  - 7
DP  - 2012 Jul
TI  - Three sensitive assays do not provide evidence for circulating HuD-specific T 
      cells in the blood of patients with paraneoplastic neurological syndromes with 
      anti-Hu antibodies.
PG  - 841-8
LID - 10.1093/neuonc/nos118 [doi]
AB  - Anti-Hu antibody-associated paraneoplastic neurological syndromes (Hu-PNSs) are 
      severe and often precede the detection of a malignancy, usually small-cell lung 
      cancer. In Hu-PNS, it is hypothesized that neuronal cells are destroyed by T 
      cells targeted against HuD, a protein expressed by small-cell lung cancer cells 
      and neurons. There is only limited evidence for the existence of HuD-specific T 
      cells. To detect these T cells in the blood of Hu-PNS patients, we employed 3 
      highly sensitive assays that included T cell stimulation with dendritic cells 
      (DCs) to specifically expand the number of any HuD-specific T cells. A total of 
      17 Hu-PNS patients were tested with 1 or more of the following 3 assays: (1) 
      tetramer staining after stimulation of T cells with conventionally generated DCs 
      (n = 9), (2) interleukin (IL)-13 enzyme-linked immunosorbent spot (ELISpot; n = 
      3), IL-4 and IL-5 and interferon (IFN)-gamma multiplex cytokine bead array (n = 2) to 
      assay cytokine production by T cells after stimulation with conventionally 
      generated DCs, and (iii) IFN-gamma ELISpot and tetramer staining after T cell 
      stimulation with accelerated co-cultured DCs (n = 11). No circulating 
      HuD-specific T cells were found. We suggest that either autoaggressive T cells in 
      Hu-PNS are not targeted against HuD or that their numbers in the blood are too 
      low for detection by highly sensitive techniques.
FAU - de Jongste, Adriaan H C
AU  - de Jongste AH
AD  - Department of Neurology, Erasmus University Medical Center, 's-Gravendijkwal 230, 
      3015 CE Rotterdam, the Netherlands.
FAU - de Graaf, Marieke T
AU  - de Graaf MT
FAU - Martinuzzi, Emanuela
AU  - Martinuzzi E
FAU - van den Broek, Patricia D M
AU  - van den Broek PD
FAU - Kraan, Jaco
AU  - Kraan J
FAU - Lamers, Cor H J
AU  - Lamers CH
FAU - Mallone, Roberto
AU  - Mallone R
FAU - Gratama, Jan W
AU  - Gratama JW
FAU - Sillevis Smitt, Peter A E
AU  - Sillevis Smitt PA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120515
PL  - England
TA  - Neuro Oncol
JT  - Neuro-oncology
JID - 100887420
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (ELAV Proteins)
RN  - 0 (Peptide Fragments)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Aged
MH  - Antibodies, Anti-Idiotypic/*blood
MH  - *Biological Assay
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Case-Control Studies
MH  - ELAV Proteins/blood/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - Middle Aged
MH  - Paraneoplastic Syndromes, Nervous System/*blood/*immunology
MH  - Peptide Fragments/immunology
MH  - T-Lymphocyte Subsets/immunology
PMC - PMC3379804
EDAT- 2012/05/18 06:00
MHDA- 2012/10/19 06:00
PMCR- 2013/07/01
CRDT- 2012/05/18 06:00
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/10/19 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - nos118 [pii]
AID - 10.1093/neuonc/nos118 [doi]
PST - ppublish
SO  - Neuro Oncol. 2012 Jul;14(7):841-8. doi: 10.1093/neuonc/nos118. Epub 2012 May 15.