PMID- 22591718
OWN - NLM
STAT- MEDLINE
DCOM- 20130523
LR  - 20220330
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 5
DP  - 2012 May 16
TI  - RASSF1C modulates the expression of a stem cell renewal gene, PIWIL1.
PG  - 239
LID - 10.1186/1756-0500-5-239 [doi]
AB  - BACKGROUND: RASSF1A and RASSF1C are two major isoforms encoded by the Ras 
      association domain family 1 (RASSF1) gene through alternative promoter selection 
      and mRNA splicing. RASSF1A is a well established tumor suppressor gene. Unlike 
      RASSF1A, RASSF1C appears to have growth promoting actions in lung cancer. In this 
      article, we report on the identification of novel RASSF1C target genes in non 
      small cell lung cancer (NSCLC). METHODS: Over-expression and siRNA techniques 
      were used to alter RASSF1C expression in human lung cancer cells, and 
      Affymetrix-microarray study was conducted using NCI-H1299 cells over-expressing 
      RASSF1C to identify RASSF1C target genes. RESULTS: The microarray study 
      intriguingly shows that RASSF1C modulates the expression of a number of genes 
      that are involved in cancer development, cell growth and proliferation, cell 
      death, and cell cycle. We have validated the expression of some target genes 
      using qRT-PCR. We demonstrate that RASSF1C over-expression increases, and 
      silencing of RASSF1C decreases, the expression of PIWIL1 gene in NSCLC cells 
      using qRT-PCR, immunostaining, and Western blot analysis. We also show that 
      RASSF1C over-expression induces phosphorylation of ERK1/2 in lung cancer cells, 
      and inhibition of the MEK-ERK1/2 pathway suppresses the expression of PIWIL1 gene 
      expression, suggesting that RASSF1C may exert its activities on some target genes 
      such as PIWIL1 through the activation of the MEK-ERK1/2 pathway. Also, PIWIL1 
      expression is elevated in lung cancer cell lines compared to normal lung 
      epithelial cells. CONCLUSIONS: Taken together, our findings provide significant 
      data to propose a model for investigating the role of RASSF1C/PIWIL1 proteins in 
      initiation and progression of lung cancer.
FAU - Reeves, Mark E
AU  - Reeves ME
AD  - Surgical Oncology Laboratory, Loma Linda VA Medical Center, CA 92357, USA.
FAU - Baldwin, Melissa L
AU  - Baldwin ML
FAU - Aragon, Robert
AU  - Aragon R
FAU - Baldwin, Scott
AU  - Baldwin S
FAU - Chen, Shin-Tai
AU  - Chen ST
FAU - Li, Xinmin
AU  - Li X
FAU - Mohan, Subburaman
AU  - Mohan S
FAU - Amaar, Yousef G
AU  - Amaar YG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120516
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (2-(2-chloro-4-iodophenylamino)-N-cyclopropylmethoxy-3,4-difluorobenzamide)
RN  - 0 (Argonaute Proteins)
RN  - 0 (Benzamides)
RN  - 0 (PIWIL1 protein, human)
RN  - 0 (RASSF1 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Apoptosis/genetics
MH  - Argonaute Proteins/*genetics/metabolism
MH  - Benzamides/pharmacology
MH  - Blotting, Western
MH  - Carcinoma, Non-Small-Cell Lung/genetics/metabolism/pathology
MH  - Cell Cycle/genetics
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic/drug effects/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung Neoplasms/genetics/metabolism/pathology
MH  - MAP Kinase Signaling System/drug effects
MH  - Neoplastic Stem Cells/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phosphorylation/drug effects
MH  - RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Suppressor Proteins/*genetics/metabolism
PMC - PMC3512503
EDAT- 2012/05/18 06:00
MHDA- 2013/05/25 06:00
PMCR- 2012/05/16
CRDT- 2012/05/18 06:00
PHST- 2011/10/13 00:00 [received]
PHST- 2012/04/02 00:00 [accepted]
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2013/05/25 06:00 [medline]
PHST- 2012/05/16 00:00 [pmc-release]
AID - 1756-0500-5-239 [pii]
AID - 10.1186/1756-0500-5-239 [doi]
PST - epublish
SO  - BMC Res Notes. 2012 May 16;5:239. doi: 10.1186/1756-0500-5-239.