PMID- 22591855
OWN - NLM
STAT- MEDLINE
DCOM- 20121210
LR  - 20120529
IS  - 1464-3391 (Electronic)
IS  - 0968-0896 (Linking)
VI  - 20
IP  - 12
DP  - 2012 Jun 15
TI  - Targeting of polyplex to human hepatic cells by bio-nanocapsules, hepatitis B 
      virus surface antigen L protein particles.
PG  - 3873-9
LID - 10.1016/j.bmc.2012.04.031 [doi]
AB  - We have previously demonstrated that lipoplex, a complex of cationic liposomes 
      and DNA, could be targeted to human hepatic cells in vitro and in vivo by 
      conjugation with bio-nanocapsules (BNCs) comprising hepatitis B virus (HBV) 
      surface antigen L protein particles. Because the BNC-lipoplex complexes were 
      endowed with the human hepatic cell-specific infection machinery from HBV, the 
      complexes showed excellent specific transfection efficiency in human hepatic 
      cells. In this study, we have found that polyplex (a complex of polyethyleneimine 
      (PEI) and DNA) could form stable complexes with BNCs spontaneously. The diameter 
      and zeta-potential of BNC-polyplex complexes are about 240 nm and +3.54 mV, 
      respectively, which make them more suitable for in vivo use than polyplex alone. 
      BNC-polyplex complexes with an N/P ratio (the molar ratio of the amine group of 
      PEI to the phosphate group of DNA) of 40 showed excellent transfection efficiency 
      in human hepatic cells. When acidification of endosomes was inhibited by 
      bafilomycin A1, the complexes showed higher transfection efficiency than polyplex 
      itself, strongly suggesting that the complexes escaped from endosomes by both 
      fusogenic activity of BNCs and proton sponge activity of polyplex. Furthermore, 
      the cytotoxicity is comparable to that of polyplex of the same N/P value. Thus, 
      BNC-polyplex complexes would be a promising gene delivery carrier for human 
      liver-specific gene therapy.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Somiya, Masaharu
AU  - Somiya M
AD  - Department of Bioengineering Sciences, Graduate School of Bioagricultural 
      Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan.
FAU - Yoshimoto, Nobuo
AU  - Yoshimoto N
FAU - Iijima, Masumi
AU  - Iijima M
FAU - Niimi, Tomoaki
AU  - Niimi T
FAU - Dewa, Takehisa
AU  - Dewa T
FAU - Jung, Joohee
AU  - Jung J
FAU - Kuroda, Shun'ichi
AU  - Kuroda S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120421
PL  - England
TA  - Bioorg Med Chem
JT  - Bioorganic & medicinal chemistry
JID - 9413298
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (Macrolides)
RN  - 88899-55-2 (bafilomycin A1)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*chemistry
MH  - Dose-Response Relationship, Drug
MH  - Endosomes/drug effects
MH  - *Gene Transfer Techniques
MH  - Genetic Therapy
MH  - Hepatitis B Surface Antigens/*chemistry/metabolism
MH  - Hepatocytes/*metabolism
MH  - Humans
MH  - Macrolides/pharmacology
MH  - Nanoparticles/*chemistry
MH  - Organ Specificity
MH  - Particle Size
MH  - Polyethyleneimine/*chemistry/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2012/05/18 06:00
MHDA- 2012/12/12 06:00
CRDT- 2012/05/18 06:00
PHST- 2012/02/09 00:00 [received]
PHST- 2012/04/15 00:00 [revised]
PHST- 2012/04/16 00:00 [accepted]
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/12/12 06:00 [medline]
AID - S0968-0896(12)00315-X [pii]
AID - 10.1016/j.bmc.2012.04.031 [doi]
PST - ppublish
SO  - Bioorg Med Chem. 2012 Jun 15;20(12):3873-9. doi: 10.1016/j.bmc.2012.04.031. Epub 
      2012 Apr 21.