PMID- 22592193
OWN - NLM
STAT- MEDLINE
DCOM- 20120724
LR  - 20211203
IS  - 0172-780X (Print)
IS  - 0172-780X (Linking)
VI  - 33
IP  - 2
DP  - 2012
TI  - An active product of cruciferous vegetables, 3,3'-diindolylmethane, inhibits 
      invasive properties of extravillous cytotrophoblastic cells.
PG  - 133-7
AB  - OBJECTIVES: During implantation, human trophoblastic cells have to proliferate, 
      migrate and invade pregnant uterus. A natural product of cruciferous vegetables, 
      3,3'-diindolylmethane (DIM), is known to induce some stress response genes (such 
      as glucose-regulated protein 78 kDa (GRP78)) and to have anti-invasive and 
      pro-apoptotic effects on tumor cells. Therefore, we have investigated the 
      potential effect of DIM on invasive extravillous cytotrophoblasts (evCTBs) cells. 
      MATERIALS AND METHODS: evCTBs were purified from first trimester trophoblasts and 
      cultured in presence or not of DIM for 48h. In order to evaluate invasive 
      properties of cells, they were seeded on collagen-coated insert following boyden 
      chamber principle and matrix metalloproteinases (MMPs) and GRP78 expression was 
      evaluated by qPCR. RESULTS: We showed that DIM decreases (p=0.013) invasive 
      properties of evCTBs. In parallel, we determined that MMP-2, -7 and -9 which are 
      involved in evCTBs invasion and known to be regulated by DIM, are not affected by 
      DIM in evCTBs. In contrast, MMP-1 mRNA is induced (p=0.03) and MMP-12 is 
      decreased (p=0.01) in DIM treated cells. Moreover, DIM treatment does not affect 
      GRP78 mRNA expression in evCTBs. CONCLUSIONS: Collectively, the present results 
      provide evidence that DIM does not impact evenly on evCTBs and cancer cells.
FAU - Ribaux, Pascale
AU  - Ribaux P
AD  - Laboratoire d'Hormonologie, Department of Gynaecology and Obstetrics, HUG, 
      Maternite, Geneva, Switzerland.
FAU - Irion, Olivier
AU  - Irion O
FAU - Cohen, Marie
AU  - Cohen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Neuro Endocrinol Lett
JT  - Neuro endocrinology letters
JID - 8008373
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Indoles)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - SSZ9HQT61Z (3,3'-diindolylmethane)
SB  - IM
MH  - Anticarcinogenic Agents/*pharmacology
MH  - Cell Movement/*drug effects
MH  - Cells, Cultured
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Female
MH  - Gene Expression/drug effects
MH  - Heat-Shock Proteins/biosynthesis
MH  - Humans
MH  - Indoles/*pharmacology
MH  - Matrix Metalloproteinases/biosynthesis
MH  - Pregnancy
MH  - Trophoblasts/*drug effects/*metabolism
EDAT- 2012/05/18 06:00
MHDA- 2012/07/25 06:00
CRDT- 2012/05/18 06:00
PHST- 2012/01/13 00:00 [received]
PHST- 2012/03/18 00:00 [accepted]
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/07/25 06:00 [medline]
AID - NEL330212A01 [pii]
PST - ppublish
SO  - Neuro Endocrinol Lett. 2012;33(2):133-7.