PMID- 2259229
OWN - NLM
STAT- MEDLINE
DCOM- 19910128
LR  - 20190824
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 14
IP  - 10
DP  - 1990
TI  - Flow cytometric studies on actin polymerization in PMN cells from chronic myeloid 
      leukemia (CML) patients.
PG  - 921-30
AB  - Studies in our laboratory have shown that polymorphonuclear leucocytes (PMNL) 
      from chronic myeloid leukemia (CML) patients are defective in chemotaxis towards 
      a synthetic peptide, n-formyl-methionyl-leucyl-phenylalanine (FMLP), during the 
      active phases of the disease and in remission. Actin plays a major role in 
      cellular movements and binding of chemo-attractant to cells induces 
      polymerization of G-actin to F-actin. We have, therefore, compared polymerization 
      of actin in FMLP stimulated PMNL from CML patients with those from normal 
      subjects by fluorescence microscopy and flow cytometry, using F-actin specific 
      probe, NBD-phallacidin. Our results show that binding of FMLP to normal PMNL 
      induces rapid conversion of G-actin to F-actin followed by depolymerization to 
      some extent. In CML PMNL, such a biphasic response is not seen. Conversion of 
      G-actin to F-actin is slower and F-actin content is significantly lower than that 
      in normal PMNL. Moreover, organization of F-actin is different in CML PMNL as 
      compared to that in normal PMNL.
FAU - Naik, N R
AU  - Naik NR
AD  - Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
FAU - Bhisey, A N
AU  - Bhisey AN
FAU - Advani, S H
AU  - Advani SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Actins)
RN  - 0 (Amanitins)
RN  - 0 (Polymers)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 73413-78-2 (7-nitrobenz-2-oxa-1,3-diazole-phallacidin)
SB  - IM
MH  - Actins/*metabolism
MH  - Amanitins
MH  - Flow Cytometry
MH  - Humans
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*blood
MH  - Microscopy, Fluorescence
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism/pharmacology
MH  - Neutrophils/*metabolism
MH  - Polymers
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1016/0145-2126(90)90183-a [doi]
PST - ppublish
SO  - Leuk Res. 1990;14(10):921-30. doi: 10.1016/0145-2126(90)90183-a.