PMID- 22592910
OWN - NLM
STAT- MEDLINE
DCOM- 20130412
LR  - 20211021
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 44
IP  - 8
DP  - 2012 Dec
TI  - Detection of occurrence of a recent genetic bottleneck event in Indian hill 
      cattle breed Bargur using microsatellite markers.
PG  - 2007-13
LID - 10.1007/s11250-012-0171-8 [doi]
AB  - The effective number of breedable individuals is a crucial determinant for 
      maintaining genetic variability within a population. The population of Bargur, 
      the hill cattle of South India, has gone down drastically by more than 93 % in 
      the past three decades, and only a few thousand animals are available at present. 
      The present study was undertaken to evaluate Bargur cattle for mutation drift 
      equilibrium and to detect the occurrence of recent genetic bottleneck event, if 
      any, in this population. About 50 unrelated animals, true to the type, were 
      sampled and genotyped at 25 microsatellite loci. The mean observed heterozygosity 
      (0.808 +/- 0.023) was higher than the mean expected heterozygosity (0.762 +/- 0.008) 
      with 15 out of 25 microsatellite loci exhibiting heterozygosity excess when 
      assumed under Hardy-Weinberg equilibrium. To evaluate Bargur cattle for mutation 
      drift equilibrium, three tests were performed under three different mutation 
      models, viz., infinite allele model (IAM), stepwise mutation model (SMM) and 
      two-phase model (TPM). The observed gene diversity (H (e)) and expected 
      equilibrium gene diversity (H(eq)) were estimated under different models of 
      microsatellite evolution. All the 25 loci were found to exhibit gene diversity 
      excess under IAM and TPM, while 22 loci were having gene diversity excess under 
      SMM. All the three statistical tests, viz., sign test, standardized differences 
      test, and Wilcoxon sign rank test, revealed significant (P < 0.01) deviation of 
      Bargur cattle population from mutation-drift equilibrium under all the three 
      models of mutation. Furthermore, the qualitative test of allele frequency 
      distribution in Bargur cattle population revealed a strong mode shift from the 
      normal L-shaped form suggesting that the population had experienced genetic 
      bottleneck in the recent past. The occurrence of genetic bottleneck might have 
      led to the loss of several rare alleles in the population, which point towards 
      the need for efforts to conserve this important cattle germplasm. The present 
      study is the first report in demonstrating the genetic basis of demographic 
      bottleneck in an Indian cattle population.
FAU - Ganapathi, Palanisamy
AU  - Ganapathi P
AD  - Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, 
      Chennai, India.
FAU - Rajendran, Ramanujam
AU  - Rajendran R
FAU - Kathiravan, Periasamy
AU  - Kathiravan P
LA  - eng
PT  - Journal Article
DEP - 20120518
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
SB  - IM
MH  - Animals
MH  - Cattle/*genetics
MH  - *Genetic Drift
MH  - Genotype
MH  - India
MH  - Male
MH  - *Microsatellite Repeats
MH  - Models, Biological
MH  - Multilocus Sequence Typing/veterinary
MH  - Mutation
MH  - Polymerase Chain Reaction/veterinary
EDAT- 2012/05/18 06:00
MHDA- 2013/04/13 06:00
CRDT- 2012/05/18 06:00
PHST- 2012/05/04 00:00 [accepted]
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2013/04/13 06:00 [medline]
AID - 10.1007/s11250-012-0171-8 [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2012 Dec;44(8):2007-13. doi: 10.1007/s11250-012-0171-8. 
      Epub 2012 May 18.