PMID- 22593018
OWN - NLM
STAT- MEDLINE
DCOM- 20121129
LR  - 20211021
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 30
IP  - 6
DP  - 2012 Jun
TI  - Enhanced homing permeability and retention of bone marrow stromal cells by 
      noninvasive pulsed focused ultrasound.
PG  - 1216-27
LID - 10.1002/stem.1099 [doi]
AB  - Bone marrow stromal cells (BMSCs) have shown significant promise in the treatment 
      of disease, but their therapeutic efficacy is often limited by inefficient homing 
      of systemically administered cells, which results in low number of cells 
      accumulating at sites of pathology. BMSC home to areas of inflammation where 
      local expression of integrins and chemokine gradients is present. We demonstrated 
      that nondestructive pulsed focused ultrasound (pFUS) exposures that emphasize the 
      mechanical effects of ultrasound-tissue interactions induced local and transient 
      elevations of chemoattractants (i.e., cytokines, integrins, and growth factors) 
      in the murine kidney. pFUS-induced upregulation of cytokines occurred through 
      approximately 1 day post-treatment and returned to contralateral kidney levels by 
      day 3. This window of significant increases in cytokine expression was 
      accompanied by local increases of other trophic factors and integrins that have 
      been shown to promote BMSC homing. When BMSCs were intravenously administered 
      following pFUS treatment to a single kidney, enhanced homing, permeability, and 
      retention of BMSC was observed in the treated kidney versus the contralateral 
      kidney. Histological analysis revealed up to eight times more BMSC in the 
      peritubular regions of the treated kidneys on days 1 and 3 post-treatment. 
      Furthermore, cytokine levels in pFUS-treated kidneys following BMSC 
      administration were found to be similar to controls, suggesting modulation of 
      cytokine levels by BMSC. pFUS could potentially improve cell-based therapies as a 
      noninvasive modality to target homing by establishing local chemoattractant 
      gradients and increasing expression of integrins to enhance tropism of cells 
      toward treated tissues.
CI  - Copyright (c) 2012 AlphaMed Press.
FAU - Ziadloo, Ali
AU  - Ziadloo A
AD  - Laboratory of Diagnostic Radiology Research, Radiology and Imaging Sciences, 
      Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA.
FAU - Burks, Scott R
AU  - Burks SR
FAU - Gold, Eric M
AU  - Gold EM
FAU - Lewis, Bobbi K
AU  - Lewis BK
FAU - Chaudhry, Aneeka
AU  - Chaudhry A
FAU - Merino, Maria J
AU  - Merino MJ
FAU - Frenkel, Victor
AU  - Frenkel V
FAU - Frank, Joseph A
AU  - Frank JA
LA  - eng
GR  - ZIA CL040014-03/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/*diagnostic imaging/metabolism
MH  - Bone Marrow Transplantation/diagnostic imaging/*methods
MH  - Cell Culture Techniques
MH  - Cytokines/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney/*cytology/*diagnostic imaging
MH  - Mesenchymal Stem Cells/cytology/diagnostic imaging
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Stromal Cells/cytology/*transplantation
MH  - Ultrasonics/*methods
MH  - Ultrasonography
PMC - PMC3356926
MID - NIHMS363906
COIS- Disclosure of Potential Conflicts of Interest The authors indicate no potential 
      conflicts of interest.
EDAT- 2012/05/18 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/06/01
CRDT- 2012/05/18 06:00
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - 10.1002/stem.1099 [doi]
PST - ppublish
SO  - Stem Cells. 2012 Jun;30(6):1216-27. doi: 10.1002/stem.1099.