PMID- 22593050
OWN - NLM
STAT- MEDLINE
DCOM- 20120727
LR  - 20211021
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 32
IP  - 20
DP  - 2012 May 16
TI  - Egr-1 induces DARPP-32 expression in striatal medium spiny neurons via a 
      conserved intragenic element.
PG  - 6808-18
LID - 10.1523/JNEUROSCI.5448-11.2012 [doi]
AB  - DARPP-32 (dopamine and adenosine 3', 5'-cyclic monophosphate cAMP-regulated 
      phosphoprotein, 32 kDa) is a striatal-enriched protein that mediates signaling by 
      dopamine and other first messengers in the medium spiny neurons. The 
      transcriptional mechanisms that regulate striatal DARPP-32 expression remain 
      enigmatic and are a subject of much interest in the efforts to induce a striatal 
      phenotype in stem cells. We report the identification and characterization of a 
      conserved region, also known as H10, in intron IV of the gene that codes for 
      DARPP-32 (Ppp1r1b). This DNA sequence forms multiunit complexes with nuclear 
      proteins from adult and embryonic striata of mice and rats. Purification of 
      proteins from these complexes identified early growth response-1 (Egr-1). The 
      interaction between Egr-1 and H10 was confirmed in vitro and in vivo by 
      super-shift and chromatin immunoprecipitation assays, respectively. Importantly, 
      brain-derived neurotrophic factor (BDNF), a known inducer of DARPP-32 and Egr-1 
      expression, enhanced Egr-1 binding to H10 in vitro. Moreover, overexpression of 
      Egr-1 in primary striatal neurons induced the expression of DARPP-32, whereas a 
      dominant-negative Egr-1 blocked DARPP-32 induction by BDNF. Together, this study 
      identifies Egr-1 as a transcriptional activator of the Ppp1r1b gene and provides 
      insight into the molecular mechanisms that regulate medium spiny neuron 
      maturation.
FAU - Keilani, Serene
AU  - Keilani S
AD  - Department of Neurology and Pediatrics, Mount Sinai School of Medicine, New York, 
      New York 10029, USA.
FAU - Chandwani, Samira
AU  - Chandwani S
FAU - Dolios, Georgia
AU  - Dolios G
FAU - Bogush, Alexey
AU  - Bogush A
FAU - Beck, Heike
AU  - Beck H
FAU - Hatzopoulos, Antonis K
AU  - Hatzopoulos AK
FAU - Rao, Gadiparthi N
AU  - Rao GN
FAU - Thomas, Elizabeth A
AU  - Thomas EA
FAU - Wang, Rong
AU  - Wang R
FAU - Ehrlich, Michelle E
AU  - Ehrlich ME
LA  - eng
GR  - NS-045942/NS/NINDS NIH HHS/United States
GR  - R01 HL103575/HL/NHLBI NIH HHS/United States
GR  - P30 NS061777/NS/NINDS NIH HHS/United States
GR  - HL103575/HL/NHLBI NIH HHS/United States
GR  - R01 EY014856/EY/NEI NIH HHS/United States
GR  - S10 RR022415/RR/NCRR NIH HHS/United States
GR  - EY014856/EY/NEI NIH HHS/United States
GR  - R01 NS045942/NS/NINDS NIH HHS/United States
GR  - R01 HL069908/HL/NHLBI NIH HHS/United States
GR  - HL069908/HL/NHLBI NIH HHS/United States
GR  - R01 NS059936/NS/NINDS NIH HHS/United States
GR  - NS-059936/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dopamine and cAMP-Regulated Phosphoprotein 32)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/pharmacology
MH  - Corpus Striatum/drug effects/*metabolism
MH  - Dopamine and cAMP-Regulated Phosphoprotein 32/*biosynthesis/*genetics
MH  - Early Growth Response Protein 1/*metabolism
MH  - Gene Expression Regulation/drug effects/*genetics
MH  - Introns/*genetics
MH  - Mice
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/metabolism
MH  - Primary Cell Culture
MH  - Protein Binding/genetics
MH  - Rats
MH  - Sequence Alignment/methods
MH  - Transcription Factors/*metabolism
PMC - PMC3752065
EDAT- 2012/05/18 06:00
MHDA- 2012/07/28 06:00
PMCR- 2012/11/16
CRDT- 2012/05/18 06:00
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/07/28 06:00 [medline]
PHST- 2012/11/16 00:00 [pmc-release]
AID - 32/20/6808 [pii]
AID - 3773037 [pii]
AID - 10.1523/JNEUROSCI.5448-11.2012 [doi]
PST - ppublish
SO  - J Neurosci. 2012 May 16;32(20):6808-18. doi: 10.1523/JNEUROSCI.5448-11.2012.