PMID- 22593432
OWN - NLM
STAT- MEDLINE
DCOM- 20121206
LR  - 20211021
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 27
IP  - 8
DP  - 2012 Aug
TI  - Amphiregulin promotes the maturation of oocytes isolated from the small antral 
      follicles of the rhesus macaque.
PG  - 2430-7
LID - 10.1093/humrep/des158 [doi]
AB  - BACKGROUND: In non-primates, the epidermal growth factor (EGF) and EGF-related 
      ligands such as amphiregulin (AREG) serve as critical intermediates between the 
      theca/mural cells and the cumulus-oocyte-complex (COC) following the mid-cycle LH 
      surge. Studies were designed in primates (1) to analyze AREG levels in follicular 
      fluid (follicular fluid) obtained from pre-ovulatory follicles, as well as (2) to 
      assess dose-dependent effects of AREG on oocytes from small antral follicles 
      (SAFs) during culture, including meiotic and cytoplasmic maturation. METHODS: 
      Controlled ovulation protocols were performed on rhesus monkeys (n=12) to 
      determine AREG content within the single, naturally selected dominant follicle 
      after an ovulatory stimulus. Using healthy COCs (n=271) obtained from SAFs during 
      spontaneous cycles (n=27), in vitro maturation (IVM) was performed in the absence 
      or presence of physiological concentrations of AREG (10 or 100 ng/ml) with or 
      without gonadotrophins (FSH, 75 mIU/ml; LH, 75 mIU/ml). At the end of the culture 
      period, oocyte meiotic maturation was evaluated and ICSI was performed (n=111), 
      from which fertilization and early embryo development was followed in vitro. 
      RESULTS: AREG levels in follicular fluid from pre-ovulatory follicles increased 
      (P<0.05) following an ovulatory bolus of hCG at 12, 24 and 36 h post-treatment. 
      At 12 h post-hCG, AREG levels in follicular fluid ranged from 4.8 to 121.4 ng/ml. 
      Rhesus macaque COCs incubated with 10 ng/ml AREG in the presence of 
      gonadotrophins displayed an increased percentage of oocytes that progressed to 
      the metaphase II (MII) stage of meiosis (82 versus 56%, P<0.05) and a decreased 
      percentage of metaphase I (MI) oocytes (2 versus 23%, P<0.05) relative to 
      controls, respectively. The percentage of either MI or MII oocytes at the end of 
      the culture period was not different between oocytes cultured with 100 ng/ml AREG 
      or in media alone. Fertilization and first cleavage rates obtained by ICSI of all 
      IVM MII oocytes were 93 and 98%, respectively, and did not vary among treatment 
      groups. Of the MII oocytes that fertilized (n=103), 37 were randomly selected and 
      maintained in culture to assess developmental potential. A total of 13 early 
      blastocysts were obtained, with four embryos developing to expanded blastocysts. 
      CONCLUSIONS: These data indicate that AREG levels increase in rhesus macaque 
      pre-ovulatory follicles after an ovulatory stimulus, and a specific concentration 
      of AREG (10 ng/ml) enhances rhesus macaque oocyte nuclear maturation but not 
      cytoplasmic maturation from SAFs obtained during the natural menstrual cycle. 
      However, owing to the small number of samples in some treatment groups, further 
      studies are now required.
FAU - Peluffo, Marina C
AU  - Peluffo MC
AD  - Centro de Investigaciones Endocrinologicas (CEDIE-CONICET), Hospital de Ninos 
      Ricardo Gutierrez, Gallo 1330, C1425SEFD Ciudad Autonoma de Buenos Aires, 
      Argentina.
FAU - Ting, Alison Y
AU  - Ting AY
FAU - Zamah, Alberuni M
AU  - Zamah AM
FAU - Conti, Marco
AU  - Conti M
FAU - Stouffer, Richard L
AU  - Stouffer RL
FAU - Zelinski, Mary B
AU  - Zelinski MB
FAU - Hennebold, Jon D
AU  - Hennebold JD
LA  - eng
GR  - U54 HD55744/HD/NICHD NIH HHS/United States
GR  - R01-HD058294/HD/NICHD NIH HHS/United States
GR  - S10RR024585/RR/NCRR NIH HHS/United States
GR  - R01 HD052909/HD/NICHD NIH HHS/United States
GR  - RR000163/RR/NCRR NIH HHS/United States
GR  - U54 HD18185/HD/NICHD NIH HHS/United States
GR  - 1 UL1 RR024926/RR/NCRR NIH HHS/United States
GR  - PL1-EB008542/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120516
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Amphiregulin)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Ligands)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Blastocyst/cytology
MH  - Cells, Cultured
MH  - Cytoplasm/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Follicular Fluid/metabolism
MH  - Glycoproteins/*physiology
MH  - Intercellular Signaling Peptides and Proteins/*physiology
MH  - Ligands
MH  - Luteinizing Hormone/metabolism
MH  - Macaca mulatta
MH  - Meiosis
MH  - Oocytes/*cytology
MH  - Oogenesis
MH  - Ovarian Follicle/cytology
MH  - Ovary/physiology
MH  - Time Factors
PMC - PMC3398676
EDAT- 2012/05/18 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/08/01
CRDT- 2012/05/18 06:00
PHST- 2012/05/18 06:00 [entrez]
PHST- 2012/05/18 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - des158 [pii]
AID - 10.1093/humrep/des158 [doi]
PST - ppublish
SO  - Hum Reprod. 2012 Aug;27(8):2430-7. doi: 10.1093/humrep/des158. Epub 2012 May 16.