PMID- 22594109
OWN - NLM
STAT- MEDLINE
DCOM- 20120717
LR  - 20191027
IS  - 0255-2922 (Print)
IS  - 0255-2922 (Linking)
VI  - 32
IP  - 1
DP  - 2012 Mar
TI  - Combinatorial effects of naomai yihao capsules and vascular endothelial growth 
      factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in 
      cerebral ischemic tissues in rats.
PG  - 87-92
AB  - OBJECTIVE: To investigate the combinatorial effects of Naomai Yihao (NMYH) 
      Capsules and vascular endothelial growth factor (VEGF) gene-transfected bone 
      marrow mesenchymal stem cells (BMSCs) on angiogenesis in cerebral ischemic 
      tissues in rats and the mechanism. METHOD: BMSCs were isolated and cultured from 
      bone marrow by an adherence method. Then, BMSCs were transfected with the 
      eukaryotic expression plasmid pEGFP-VEGF165 by positive ionic liposome 
      transfection. A rat model of middle cerebral artery occlusion (MCAO) was 
      established. Rats were allocated to six groups: model, BMSC, VEGF 
      gene-transfected BMSC transplantation (BMSC/VEGF), NMYH, combined NMYH and 
      BMSC/VEGF (combined treatment group) and sham operation groups. The behavioral 
      rating score (BRS) of rats and the expression of CD34 and VEGF in brain tissue 
      were measured by immunohistochemistry on days 7, 14 and 21 after reperfusion. 
      Angiogenesis was observed and evaluated with laser scanning confocal microscopy. 
      RESULTS: The BRS of rats in NMYH, BMSC transplantation and combined treatment 
      groups was significantly lower than that of the model group (P < 0.001), with no 
      significant difference between NMYH and transplantation groups (P = 0.619). The 
      expression of CD34 and VEGF in NMYH, transplantation and combined treatment 
      groups increased (P < 0.001), with a significant difference between NMYH and 
      transplantation groups (P < 0.001). The blood vessel area in NMYH, 
      transplantation and combined treatment groups was significantly increased (P < 
      0.05), without a significant difference between NMYH and transplantation groups 
      (P = 0.873). CONCLUSIONS: VEGF gene-transfected BMSCs improve angiogenesis in the 
      cerebral ischemic area. NMYH Capsules promote angiogenesis in MCAO rats treated 
      with BMSC transplantation, which show an improved BRS. The mechanism of 
      angiogenesis may be related to up-regulation of VEGF expression.
FAU - Guo, Jian-Wen
AU  - Guo JW
AD  - Guangdong Provincial Hospital, Traditional Chinese Medicine, Guangdong 510120, 
      China. drguo@yahoo.cn
FAU - Chen, Chao
AU  - Chen C
FAU - Huang, Yan
AU  - Huang Y
FAU - Li, Ben
AU  - Li B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
RN  - 0 (Capsules)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - Bone Marrow Transplantation
MH  - Brain Ischemia/drug therapy/*genetics/metabolism/*therapy
MH  - Capsules
MH  - Combined Modality Therapy
MH  - Drugs, Chinese Herbal/*administration & dosage
MH  - Female
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Neovascularization, Pathologic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transfection
MH  - Vascular Endothelial Growth Factor A/*genetics/metabolism
EDAT- 2012/05/19 06:00
MHDA- 2012/07/18 06:00
CRDT- 2012/05/19 06:00
PHST- 2012/05/19 06:00 [entrez]
PHST- 2012/05/19 06:00 [pubmed]
PHST- 2012/07/18 06:00 [medline]
AID - 10.1016/s0254-6272(12)60038-7 [doi]
PST - ppublish
SO  - J Tradit Chin Med. 2012 Mar;32(1):87-92. doi: 10.1016/s0254-6272(12)60038-7.