PMID- 22594977
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20211021
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 23
DP  - 2012 Aug
TI  - Tissue engineering the monosynaptic circuit of the stretch reflex arc with 
      co-culture of embryonic motoneurons and proprioceptive sensory neurons.
PG  - 5723-31
LID - 10.1016/j.biomaterials.2012.04.042 [doi]
AB  - The sensory circuit of the stretch reflex arc is composed of intrafusal muscle 
      fibers and their innervating proprioceptive neurons that convert mechanical 
      information regarding muscle length and tension into action potentials that 
      synapse onto the homonymous motoneurons in the ventral spinal cord which 
      innervate the extrafusal fibers of the same muscle. To date, the in vitro 
      synaptic connection between proprioceptive sensory neurons and spinal motoneurons 
      has not been demonstrated. A functional in vitro system demonstrating this 
      connection would enable the understanding of feedback by the integration of 
      sensory input into the spinal reflex arc. Here we report a co-culture of rat 
      embryonic motoneurons and proprioceptive sensory neurons from dorsal root ganglia 
      (DRG) in a defined serum-free medium on a synthetic silane substrate (DETA). 
      Furthermore, we have demonstrated functional synapse formation in the co-culture 
      by immunocytochemistry and electrophysiological analysis. This work will be 
      valuable for enabling in vitro model systems for the study of spinal motor 
      control and related pathologies such as spinal cord injury, muscular dystrophy 
      and spasticity by improving our understanding of the integration of the 
      mechanosensitive feedback mechanism.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Guo, Xiufang
AU  - Guo X
AD  - Hybrid Systems Lab, NanoScience Technology Center, University of Central Florida, 
      12424 Research Parkway, Suite 400, Orlando, FL 32826, USA.
FAU - Ayala, Jennifer E
AU  - Ayala JE
FAU - Gonzalez, Mercedes
AU  - Gonzalez M
FAU - Stancescu, Maria
AU  - Stancescu M
FAU - Lambert, Stephen
AU  - Lambert S
FAU - Hickman, James J
AU  - Hickman JJ
LA  - eng
GR  - R01 EB009429/EB/NIBIB NIH HHS/United States
GR  - R01 NS050452/NS/NINDS NIH HHS/United States
GR  - R01EB009429/EB/NIBIB NIH HHS/United States
GR  - R01NS050452/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120515
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Silanes)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques/methods
MH  - Electrophysiology
MH  - Ganglia, Spinal/*cytology/*embryology
MH  - Motor Neurons/*cytology/physiology/ultrastructure
MH  - Rats
MH  - *Reflex, Stretch
MH  - Sensory Receptor Cells/*cytology/physiology/ultrastructure
MH  - Silanes/chemistry
MH  - Synapses/ultrastructure
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/chemistry
PMC - PMC3575636
MID - NIHMS378836
EDAT- 2012/05/19 06:00
MHDA- 2012/09/18 06:00
PMCR- 2013/08/01
CRDT- 2012/05/19 06:00
PHST- 2012/02/08 00:00 [received]
PHST- 2012/04/16 00:00 [accepted]
PHST- 2012/05/19 06:00 [entrez]
PHST- 2012/05/19 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - S0142-9612(12)00464-4 [pii]
AID - 10.1016/j.biomaterials.2012.04.042 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Aug;33(23):5723-31. doi: 10.1016/j.biomaterials.2012.04.042. 
      Epub 2012 May 15.