PMID- 22595285
OWN - NLM
STAT- MEDLINE
DCOM- 20130610
LR  - 20211203
IS  - 1618-1298 (Electronic)
IS  - 0171-9335 (Linking)
VI  - 91
IP  - 9
DP  - 2012 Sep
TI  - Tumor necrosis factor-alpha (TNF-alpha)-mediated in vitro human retinal pigment 
      epithelial (RPE) cell migration mainly requires Akt/mTOR complex 1 (mTORC1), but 
      not mTOR complex 2 (mTORC2) signaling.
PG  - 728-37
LID - 10.1016/j.ejcb.2012.03.008 [doi]
AB  - When rhegmatogenous retinal detachment occurs, tumor necrosis factor-alpha 
      (TNF-alpha) among other cytokines leaks into the subretinal space, induces resident 
      retinal pigment epithelial (RPE) cells to migrate, which is the initial step of 
      proliferative vitreoretinopathy (PVR). In the current study, we aim to understand 
      how this is regulated by focusing the cellular mechanisms involved. Here we 
      identified an Akt/Tuberous sclerosis protein 2 (TSC2)/mTOR complex1 (mTORC1) 
      signaling pathway after TNF-alpha treatment to mediate RPE cell migration. 
      Suppression of mTORC1 activation, either by its inhibitor rapamycin, or by 
      activation of its suppressor AMP activated protein kinase (AMPK), inhibited 
      TNF-alpha-mediated RPE cell migration, while RNA interference (RNAi)-mediated 
      knocking-down of SIN1 or Rictor, two key components of mTOR complex 2 (mTORC2), 
      had no significant effect on TNF-alpha-induced RPE cell migration. Our data provide 
      initial evidence that TNF-alpha-mediated in vitro RPE cell migration mainly requires 
      Akt/mTORC1, but not mTORC2 signaling. The results of this study may lead to 
      indentify novel signaling targets against PVR.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - Liu, Yuan
AU  - Liu Y
AD  - The Affiliated Eye Hospital of Nanjing Medical University, Nanjing 210029, China.
FAU - Cao, Guo-Fan
AU  - Cao GF
FAU - Xue, Jinsong
AU  - Xue J
FAU - Wan, Jerry
AU  - Wan J
FAU - Wan, Yinsheng
AU  - Wan Y
FAU - Jiang, Qing
AU  - Jiang Q
FAU - Yao, Jin
AU  - Yao J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120516
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 2)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Cell Line
MH  - *Cell Movement
MH  - Humans
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Mechanistic Target of Rapamycin Complex 2
MH  - Multiprotein Complexes/metabolism
MH  - Proteins/*metabolism
MH  - Retinal Pigment Epithelium/*cytology/*metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2012/05/19 06:00
MHDA- 2013/06/12 06:00
CRDT- 2012/05/19 06:00
PHST- 2011/12/19 00:00 [received]
PHST- 2012/03/24 00:00 [revised]
PHST- 2012/03/26 00:00 [accepted]
PHST- 2012/05/19 06:00 [entrez]
PHST- 2012/05/19 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
AID - S0171-9335(12)00061-1 [pii]
AID - 10.1016/j.ejcb.2012.03.008 [doi]
PST - ppublish
SO  - Eur J Cell Biol. 2012 Sep;91(9):728-37. doi: 10.1016/j.ejcb.2012.03.008. Epub 
      2012 May 16.