PMID- 22596049
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20220317
IS  - 1939-327X (Electronic)
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 61
IP  - 9
DP  - 2012 Sep
TI  - Identification of adipose tissue dendritic cells correlated with 
      obesity-associated insulin-resistance and inducing Th17 responses in mice and 
      patients.
PG  - 2238-47
LID - 10.2337/db11-1274 [doi]
AB  - T-cell regulation in adipose tissue provides a link between inflammation and 
      insulin resistance. Because of alterations in adipose tissue T-cell composition 
      in obesity, we aimed to identify the antigen-presenting cells in adipose tissue 
      of obese mice and patients with insulin resistance. Dendritic cells (DCs) and T 
      cells were studied in mice and in two cohorts of obese patients. In lean mice, 
      only CD11c(+) DCs were detected in adipose tissue. Adoptive transfer of naive 
      CD4(+) T cells in Rag1(-/-) mice led to a predominant Th1 response in adipose 
      tissue. In contrast, during obesity DCs (human CD11c(+)CD1c(+) and mouse 
      CD11c(high)F4/80(low)) accumulated in adipose tissue. CD11c(high)F4/80(low) DCs 
      from obese mice induced Th17 differentiation. In patients, the presence of 
      CD11c(+)CD1c(+) DCs correlated with the BMI and with an elevation in Th17 cells. 
      In addition, these DCs led to ex vivo Th17 differentiation. CD1c gene expression 
      further correlated with homeostatic model assessment-insulin resistance in the 
      subcutaneous adipose tissue of obese patients. We show for the first time the 
      presence and accumulation of specific DCs in adipose tissue in mouse and human 
      obesity. These DCs were functional and could be important regulators of adipose 
      tissue inflammation by regulating the switch toward Th17 cell responses in 
      obesity-associated insulin resistance.
FAU - Bertola, Adeline
AU  - Bertola A
AD  - Universite de Nice-Sophia Antipolis, Faculte de Medecine, Nice, France.
FAU - Ciucci, Thomas
AU  - Ciucci T
FAU - Rousseau, Deborah
AU  - Rousseau D
FAU - Bourlier, Virginie
AU  - Bourlier V
FAU - Duffaut, Carine
AU  - Duffaut C
FAU - Bonnafous, Stephanie
AU  - Bonnafous S
FAU - Blin-Wakkach, Claudine
AU  - Blin-Wakkach C
FAU - Anty, Rodolphe
AU  - Anty R
FAU - Iannelli, Antonio
AU  - Iannelli A
FAU - Gugenheim, Jean
AU  - Gugenheim J
FAU - Tran, Albert
AU  - Tran A
FAU - Bouloumie, Anne
AU  - Bouloumie A
FAU - Gual, Philippe
AU  - Gual P
FAU - Wakkach, Abdelilah
AU  - Wakkach A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120517
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
SB  - IM
MH  - Adipose Tissue/*pathology/physiopathology
MH  - Adult
MH  - Animals
MH  - Cell Differentiation
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - Humans
MH  - Insulin Resistance
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Middle Aged
MH  - Obesity/*pathology/physiopathology
MH  - Obesity, Morbid/pathology
MH  - T-Lymphocytes, Regulatory/physiology
MH  - Th17 Cells/physiology
PMC - PMC3425417
EDAT- 2012/05/19 06:00
MHDA- 2012/11/06 06:00
PMCR- 2013/09/01
CRDT- 2012/05/19 06:00
PHST- 2012/05/19 06:00 [entrez]
PHST- 2012/05/19 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - db11-1274 [pii]
AID - 1274 [pii]
AID - 10.2337/db11-1274 [doi]
PST - ppublish
SO  - Diabetes. 2012 Sep;61(9):2238-47. doi: 10.2337/db11-1274. Epub 2012 May 17.