PMID- 2260275
OWN - NLM
STAT- MEDLINE
DCOM- 19910131
LR  - 20190828
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 24
IP  - 3-4
DP  - 1990 Sep
TI  - Comparison of a DNA probe, complement-fixation and indirect immunofluorescence 
      tests for diagnosing Anaplasma marginale in suspected carrier cattle.
PG  - 381-90
AB  - Most estimates of the prevalence of anaplasmosis have been based on serologic 
      data using the complement-fixation (CF) and/or card agglutination tests. Since 
      these tests are considered to be only about 50 percent reliable for detecting 
      carrier cattle in enzootically stable herds, the need for more sensitive 
      diagnostic tests is widely recognized. The objective in the present study was to 
      compare the sensitivity of the CF test with that of the indirect 
      immunofluorescence (IIF) test and a recently developed DNA probe in determining 
      the prevalence of Anaplasma marginale infection in cattle from an enzootic area. 
      The study herd consisted of 52 8-month-old steers and 13 3-year-old cows of mixed 
      beef breed. All cattle were initially tested for this comparative purpose. All 
      but one animal (one that was a positive reactor as assessed by all three tests, 
      and served as a positive control), were treated with long-acting oxytetracycline 
      in an attempt to clear any carrier infections. Each animal was then retested at 1 
      month and 2 months post-treatment (PT), in an effort to determine if the DNA 
      probe could be used to evaluate the effectiveness of the drug. Six of the 65 
      (9.2%) initial serum samples were CF positive. In contrast, 60 (92.3%) and 64 
      (98.5%) of the samples were positive as assessed with the IIF test and the DNA 
      probe, respectively. The DNA hybridization reactions varied in intensity within 
      the sample population indicating different individual levels of infection. The 
      DNA probe hybridized with two samples taken at 1 month PT, and with two different 
      samples taken at 2 months PT. The mean IIF titers were reduced at both the 1 
      month and 2 month sampling times. These results suggest that the drug did not 
      eliminate infections in all cattle. Some may have been cleared, but, in any 
      event, the drug did reduce the level of infections below the sensitivity of the 
      DNA probe and interrupted continuity of stimulation of antibody. Therefore, the 
      DNA probe and the IIF test appear to be considerably more sensitive in detecting 
      carrier infections than the CF test, and should be considered in future 
      epidemiologic studies.
FAU - Goff, W L
AU  - Goff WL
AD  - U.S. Department of Agriculture, Washington State University, Pullman 99164.
FAU - Stiller, D
AU  - Stiller D
FAU - Roeder, R A
AU  - Roeder RA
FAU - Johnson, L W
AU  - Johnson LW
FAU - Falk, D
AU  - Falk D
FAU - Gorham, J R
AU  - Gorham JR
FAU - McGuire, T C
AU  - McGuire TC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA Probes)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Anaplasma/genetics/*isolation & purification
MH  - Anaplasmosis/*diagnosis/drug therapy
MH  - Animals
MH  - Carrier State/diagnosis/drug therapy/*veterinary
MH  - Cattle
MH  - Cattle Diseases/*diagnosis/drug therapy
MH  - Complement Fixation Tests
MH  - *DNA Probes
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - Predictive Value of Tests
MH  - Tetracycline/therapeutic use
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
AID - 0378-1135(90)90185-X [pii]
AID - 10.1016/0378-1135(90)90185-x [doi]
PST - ppublish
SO  - Vet Microbiol. 1990 Sep;24(3-4):381-90. doi: 10.1016/0378-1135(90)90185-x.