PMID- 22605918
OWN - NLM
STAT- MEDLINE
DCOM- 20121213
LR  - 20211021
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 18
DP  - 2012
TI  - Androgen regulation of gene expression in human meibomian gland and conjunctival 
      epithelial cells.
PG  - 1055-67
AB  - PURPOSE: Androgens exert a significant influence on the structure, function 
      and/or pathophysiology of the meibomian gland and conjunctiva. We sought to 
      determine whether this hormone action involves the regulation of epithelial cell 
      gene expression in these tissues. METHODS: Immortalized human meibomian gland and 
      conjunctival epithelial cells were treated with placebo or dihydrotestosterone 
      (DHT) and processed for molecular biologic procedures. Gene expression was 
      evaluated with BeadChips and data were analyzed with bioinformatic and 
      statistical software. RESULTS: Androgen treatment significantly influenced the 
      expression of approximately 3,000 genes in immortalized human meibomian gland and 
      conjunctival epithelial cells. The nature of DHT action on gene activity was 
      predominantly cell-specific. Similarly, DHT exerted a significant, but primarily 
      cell-specific, influence on many gene ontologies and Kyoto Encyclopedia of Genes 
      and Genomes (KEGG) pathways. These included groups of genes related, for example, 
      to lipid dynamics, innate immunity, cell cycle, Janus kinase (JAK)-signal 
      transducer and activator of transcription (stat) cascades, oxidative 
      phosphorylation, the proteasome, and mammalian target of rapamycin (mTOR), Wnt, 
      and peroxisome proliferator-activated receptor (PPAR) signaling. CONCLUSIONS: Our 
      findings support our hypothesis that androgens regulate gene expression in human 
      meibomian gland and conjunctival epithelial cells. Our ongoing studies are 
      designed to determine whether many of these genes are translated and play a role 
      in the health and well being of the eye.
FAU - Khandelwal, Payal
AU  - Khandelwal P
AD  - Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical 
      School, 20 Staniford Street, Boston, MA 02114, USA.
FAU - Liu, Shaohui
AU  - Liu S
FAU - Sullivan, David A
AU  - Sullivan DA
LA  - eng
GR  - R01 EY005612/EY/NEI NIH HHS/United States
GR  - R01 EY05612/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120427
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Androgens)
RN  - 08J2K08A3Y (Dihydrotestosterone)
SB  - IM
MH  - Androgens/*pharmacology
MH  - Cell Line, Transformed
MH  - Conjunctiva/cytology/*drug effects/metabolism
MH  - Dihydrotestosterone/*pharmacology
MH  - Epithelial Cells/cytology/*drug effects/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Meibomian Glands/cytology/*drug effects/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Organ Specificity
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC3351406
EDAT- 2012/05/19 06:00
MHDA- 2012/12/14 06:00
PMCR- 2012/01/01
CRDT- 2012/05/19 06:00
PHST- 2012/02/02 00:00 [received]
PHST- 2012/04/24 00:00 [accepted]
PHST- 2012/05/19 06:00 [entrez]
PHST- 2012/05/19 06:00 [pubmed]
PHST- 2012/12/14 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 112 [pii]
AID - 2012MOLVIS0054 [pii]
PST - ppublish
SO  - Mol Vis. 2012;18:1055-67. Epub 2012 Apr 27.