PMID- 22606362
OWN - NLM
STAT- MEDLINE
DCOM- 20121016
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 5
DP  - 2012
TI  - Genome-wide gene amplification during differentiation of neural progenitor cells 
      in vitro.
PG  - e37422
LID - 10.1371/journal.pone.0037422 [doi]
LID - e37422
AB  - DNA sequence amplification is a phenomenon that occurs predictably at defined 
      stages during normal development in some organisms. Developmental gene 
      amplification was first described in amphibians during gametogenesis and has not 
      yet been described in humans. To date gene amplification in humans is a hallmark 
      of many tumors. We used array-CGH (comparative genomic hybridization) and FISH 
      (fluorescence in situ hybridization) to discover gene amplifications during in 
      vitro differentiation of human neural progenitor cells. Here we report a complex 
      gene amplification pattern two and five days after induction of differentiation 
      of human neural progenitor cells. We identified several amplified genes in neural 
      progenitor cells that are known to be amplified in malignant tumors. There is 
      also a striking overlap of amplified chromosomal regions between differentiating 
      neural progenitor cells and malignant tumor cells derived from astrocytes. Gene 
      amplifications in normal human cells as physiological process has not been 
      reported yet and may bear resemblance to developmental gene amplifications in 
      amphibians and insects.
FAU - Fischer, Ulrike
AU  - Fischer U
AD  - Department of Human Genetics, Saarland University, Homburg/Saar, Germany. 
      ulrike.fischer@uks.eu
FAU - Keller, Andreas
AU  - Keller A
FAU - Voss, Meike
AU  - Voss M
FAU - Backes, Christina
AU  - Backes C
FAU - Welter, Cornelius
AU  - Welter C
FAU - Meese, Eckart
AU  - Meese E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120514
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Brain Neoplasms/genetics
MH  - Cell Differentiation/*genetics
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Comparative Genomic Hybridization
MH  - *Gene Amplification
MH  - Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neural Stem Cells/*cytology/*metabolism
MH  - Neurogenesis/genetics
PMC - PMC3351388
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/05/19 06:00
MHDA- 2012/10/17 06:00
PMCR- 2012/05/14
CRDT- 2012/05/19 06:00
PHST- 2012/01/10 00:00 [received]
PHST- 2012/04/19 00:00 [accepted]
PHST- 2012/05/19 06:00 [entrez]
PHST- 2012/05/19 06:00 [pubmed]
PHST- 2012/10/17 06:00 [medline]
PHST- 2012/05/14 00:00 [pmc-release]
AID - PONE-D-12-01300 [pii]
AID - 10.1371/journal.pone.0037422 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(5):e37422. doi: 10.1371/journal.pone.0037422. Epub 2012 May 14.