PMID- 22607809
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20181201
IS  - 1879-3460 (Electronic)
IS  - 0168-1605 (Linking)
VI  - 157
IP  - 1
DP  - 2012 Jun 15
TI  - Prevalence, quantification and antimicrobial resistance of Campylobacter spp. on 
      chicken neck-skins at points of slaughter in 5 major cities located on 4 
      continents.
PG  - 102-7
LID - 10.1016/j.ijfoodmicro.2012.04.020 [doi]
AB  - Quantitative data on Campylobacter contamination of food are lacking, notably in 
      developing countries. We assessed Campylobacter contamination of chicken 
      neck-skins at points of slaughter in 5 major cities in Africa (Dakar in Senegal, 
      Yaounde in Cameroon), Oceania (Noumea in New Caledonia), the Indian Ocean 
      (Antananarivo in Madagascar) and Asia (Ho Chi Minh City (HCMC) in Vietnam. One 
      hundred and fifty slaughtered chickens were collected in each of the 5 major 
      cities from semi-industrial abattoirs or markets (direct slaughter by the 
      seller), and 65.5% (491/750) were found to be Campylobacter-positive. Two cities, 
      Yaounde and Noumea, demonstrated high prevalence Campylobacter detection rates 
      (92.7% and 96.7% respectively) in contrast with HCMC (15.3%). Four species were 
      identified among 633 isolates, namely C. jejuni (48.3%), C. coli (37.3%), C. lari 
      (11.7%) and C. upsaliensis (1%). HCMC was the only city with C. lari isolation as 
      was Antananarivo for C. upsaliensis. C. coli was highly prevalent only in Yaounde 
      (69.5%). Among the 491 samples positive in Campylobacter detection, 329 were also 
      positive with the enumeration method. The number of Campylobacter colony-forming 
      units (CFU) per gram of neck-skin in samples positive in enumeration was high 
      (mean of the log(10): 3.2 log(10) CFU/g, arithmetic mean: 7900CFU/g). All the 
      cities showed close enumeration means except HCMC with a 1.81 log(10) CFU/g mean 
      for positive samples. Semi-industrial abattoir was linked to a significant lower 
      count of Campylobacter contamination than direct slaughter by the seller 
      (p=0.006). On 546 isolates (546/633, 86.3%) tested for antibiotic susceptibility, 
      resistance to erythromycin, ampicillin and ciprofloxacin was observed for 
      respectively 11%, 19% and 50%. HCMC was the city where antibiotic resistant rates 
      were the highest (95%, p=0.014). Considering the 329 positive chickens in 
      Campylobacter enumeration, the mean number of resistant isolates to at least 2 
      different antibiotic families (19.8%), may be estimated ca. 1500CFU/g; the 
      corresponding mean of the log(10) would be 2.5 log(10)CFU/g. As chickens are sold 
      at slaughter and brought directly at home to be cooked, these data suggest a high 
      probability of cross-contamination. A substantial proportion of isolates are 
      drug-resistant, which could lead to potential public health issues. Health 
      authorities should consider measures to reduce Campylobacter contamination of 
      chicken during farming and at slaughter, and to provide appropriate food hygiene 
      education. Further studies are needed in particular to investigate food-handling 
      practices in domestic kitchens.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Garin, Benoit
AU  - Garin B
AD  - Institut Pasteur de Dakar, BP 220, Dakar, Senegal. benoitgarin@gmail.com
FAU - Gouali, Malika
AU  - Gouali M
FAU - Wouafo, Marguerite
AU  - Wouafo M
FAU - Perchec, Anne-Marie
AU  - Perchec AM
FAU - Pham, Minh Thu
AU  - Pham MT
FAU - Ravaonindrina, Noro
AU  - Ravaonindrina N
FAU - Urbes, Florence
AU  - Urbes F
FAU - Gay, Manu
AU  - Gay M
FAU - Diawara, Abdoulaye
AU  - Diawara A
FAU - Leclercq, Alexandre
AU  - Leclercq A
FAU - Rocourt, Jocelyne
AU  - Rocourt J
FAU - Pouillot, Regis
AU  - Pouillot R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120501
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Abattoirs
MH  - Africa
MH  - Animal Husbandry
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Asia
MH  - Cameroon
MH  - Campylobacter/*isolation & purification
MH  - Chickens/*microbiology
MH  - Cities
MH  - Colony Count, Microbial
MH  - Cooking
MH  - Developing Countries
MH  - Food Contamination/*statistics & numerical data
MH  - Food Handling/methods
MH  - Food Safety
MH  - Indian Ocean
MH  - Madagascar
MH  - Meat/*microbiology
MH  - New Caledonia
MH  - Oceania
MH  - Prevalence
MH  - Senegal
MH  - Vietnam
EDAT- 2012/05/23 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/05/22 06:00
PHST- 2011/07/25 00:00 [received]
PHST- 2012/02/22 00:00 [revised]
PHST- 2012/04/21 00:00 [accepted]
PHST- 2012/05/22 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
AID - S0168-1605(12)00224-3 [pii]
AID - 10.1016/j.ijfoodmicro.2012.04.020 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2012 Jun 15;157(1):102-7. doi: 
      10.1016/j.ijfoodmicro.2012.04.020. Epub 2012 May 1.