PMID- 22609353
OWN - NLM
STAT- MEDLINE
DCOM- 20120815
LR  - 20181201
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 430
IP  - 1
DP  - 2012 Aug 15
TI  - Porcine reproductive and respiratory syndrome virus induces CD4+CD8+CD25+Foxp3+ 
      regulatory T cells (Tregs).
PG  - 73-80
LID - 10.1016/j.virol.2012.04.009 [doi]
AB  - The aim of this study was to analyze the regulatory T cells (Tregs) induced by 
      the porcine reproductive and respiratory syndrome virus (PRRSV) in pigs. Serum, 
      blood, tonsil, and mediastinal lymph nodes' samples were obtained at different 
      time post-infection (dpi). The frequencies of CD4(+)CD8(-)CD25(+)Foxp3(+), 
      CD4(+)CD8(+)CD25(+)Foxp3(+), or CD4(-)CD8(+)CD25(+)Foxp3(+) phenotypes were 
      determined in PBMC and lymph node cells, and cells producing IL-10 or TGF-beta were 
      analyzed. PRRSV increased the number of CD4(+)CD8(+)CD25(+)Foxp3(+) cells at 14 
      dpi, whereas CD4(+)CD8(-)CD25(+)Foxp3(+) remained constant until 28 dpi. Positive 
      correlation exists between viremia and induced regulatory cells. 
      CD4(+)CD8(+)CD25(+)Foxp3(+)-induced Treg cells were consistently observed in 
      lymphoid tissues. Analysis of IL-10- and TGF-beta-producing cell demonstrated that 
      in response to PRRSV, CD4(+)CD8(-)Foxp3(low) and CD4(+)CD8(+)Foxp3(high) cells 
      increase moderately the proportion of IL-10(+) cells. TGF-beta was only observed in 
      the CD4(+)CD8(+)Foxp3(high) population after PRRSV stimulation. In conclusion, 
      PRRSV infection increases the frequency of Tregs with the phenotype 
      CD4(+)CD8(+)CD25(+)Foxp3(high) and produces TGF-beta.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Silva-Campa, Erika
AU  - Silva-Campa E
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo, 
      A.C. Carretera a La Victoria Km 0.6 C.P. 83304, Hermosillo, Sonora, Mexico.
FAU - Mata-Haro, Veronica
AU  - Mata-Haro V
FAU - Mateu, Enric
AU  - Mateu E
FAU - Hernandez, Jesus
AU  - Hernandez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120518
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 135845-91-9 (Hepatocyte Nuclear Factor 3-gamma)
SB  - IM
MH  - Animals
MH  - Blood/immunology
MH  - CD4 Antigens/analysis
MH  - CD8 Antigens/analysis
MH  - Hepatocyte Nuclear Factor 3-gamma/analysis
MH  - Interleukin-10/metabolism
MH  - Interleukin-2 Receptor alpha Subunit/analysis
MH  - Lymph Nodes/immunology
MH  - Palatine Tonsil/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - T-Lymphocytes, Regulatory/chemistry/*immunology/*virology
MH  - Time Factors
MH  - Transforming Growth Factor beta/metabolism
EDAT- 2012/05/23 06:00
MHDA- 2012/08/16 06:00
CRDT- 2012/05/22 06:00
PHST- 2011/12/20 00:00 [received]
PHST- 2012/01/23 00:00 [revised]
PHST- 2012/04/11 00:00 [accepted]
PHST- 2012/05/22 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/08/16 06:00 [medline]
AID - S0042-6822(12)00193-6 [pii]
AID - 10.1016/j.virol.2012.04.009 [doi]
PST - ppublish
SO  - Virology. 2012 Aug 15;430(1):73-80. doi: 10.1016/j.virol.2012.04.009. Epub 2012 
      May 18.