PMID- 22609517
OWN - NLM
STAT- MEDLINE
DCOM- 20120921
LR  - 20240318
IS  - 1879-3185 (Electronic)
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 300
IP  - 1-2
DP  - 2012 Oct 9
TI  - Developmental cigarette smoke exposure: liver proteome profile alterations in low 
      birth weight pups.
PG  - 1-11
LID - 10.1016/j.tox.2012.04.016 [doi]
AB  - Cigarette smoke is composed of over 4000 chemicals many of which are strong 
      oxidizing agents and chemical carcinogens. Chronic cigarette smoke exposure (CSE) 
      induces mild alterations in liver histology indicative of toxicity though the 
      molecular pathways underlying these alterations remain to be explored. Utilizing 
      a mouse model of 'active' developmental CSE (gestational day (GD) 1 through 
      postnatal day (PD) 21; cotinine >50ng/mL) characterized by low birth weight 
      offspring, the impact of developmental CSE on liver protein abundances was 
      determined. On PD21, liver tissue was collected from pups for 2D SDS-PAGE based 
      proteome analysis with statistical analysis by Partial Least Squares-Discriminant 
      Analysis (PLS-DA). Protein spots of interest were identified by ESI-MS/MS with 
      impacted molecular pathways identified by Ingenuity Pathway Analysis. 
      Developmental CSE decreased the abundance of proteins associated with the small 
      molecule biochemistry (includes glucose metabolism), lipid metabolism, amino acid 
      metabolism, and inflammatory response pathways. Decreased gluconeogenic enzyme 
      activity and lysophosphatidylcholine availability following developmental CSE 
      were found and supports the impact of CSE on these pathways. Proteins with 
      increased abundance belonged to the cell death and drug metabolism networks. 
      Liver antioxidant enzyme abundances [glutathione-S-transferase (GST) and 
      peroxiredoxins] were also altered by CSE, but GST enzymatic activity was 
      unchanged. In summary, cigarette smoke exposure spanning pre- and post-natal 
      development resulted in persistent decreased offspring weights, decreased 
      abundances of liver metabolic proteins, decreased gluconeogenic activity, and 
      altered lipid metabolism. The companion paper details the kidney proteome 
      alterations in the same offspring.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd. All rights reserved.
FAU - Canales, Lorena
AU  - Canales L
AD  - Department of Environmental and Occupational Health Sciences, School of Public 
      Health and Information Sciences, University of Louisville, Louisville, KY, USA.
FAU - Chen, Jing
AU  - Chen J
FAU - Kelty, Elizabeth
AU  - Kelty E
FAU - Musah, Sadiatu
AU  - Musah S
FAU - Webb, Cindy
AU  - Webb C
FAU - Pisano, M Michele
AU  - Pisano MM
FAU - Neal, Rachel E
AU  - Neal RE
LA  - eng
GR  - P30 ES014443/ES/NIEHS NIH HHS/United States
GR  - P20 GM103453/GM/NIGMS NIH HHS/United States
GR  - R21 DA027466/DA/NIDA NIH HHS/United States
GR  - P20 RR017702/RR/NCRR NIH HHS/United States
GR  - P20 RR/DE-17702/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120515
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Proteome)
RN  - 0 (Tobacco Smoke Pollution)
SB  - IM
MH  - Animals
MH  - Animals, Newborn/blood/growth & development
MH  - Disease Models, Animal
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - Gluconeogenesis/drug effects
MH  - Inhalation Exposure/adverse effects
MH  - Liver/chemistry/*drug effects/enzymology
MH  - Male
MH  - Mass Spectrometry
MH  - Metabolome/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proteome/*analysis
MH  - Tobacco Smoke Pollution/*adverse effects
PMC - PMC3699338
MID - NIHMS378672
EDAT- 2012/05/23 06:00
MHDA- 2012/09/22 06:00
PMCR- 2013/10/09
CRDT- 2012/05/22 06:00
PHST- 2010/11/01 00:00 [received]
PHST- 2012/04/23 00:00 [revised]
PHST- 2012/04/25 00:00 [accepted]
PHST- 2012/05/22 06:00 [entrez]
PHST- 2012/05/23 06:00 [pubmed]
PHST- 2012/09/22 06:00 [medline]
PHST- 2013/10/09 00:00 [pmc-release]
AID - S0300-483X(12)00163-1 [pii]
AID - 10.1016/j.tox.2012.04.016 [doi]
PST - ppublish
SO  - Toxicology. 2012 Oct 9;300(1-2):1-11. doi: 10.1016/j.tox.2012.04.016. Epub 2012 
      May 15.